Document Detail

Attenuated total reflection Fourier transform infrared spectroscopic investigation of the postmortem metabolic process in rat and human kidney cortex.
MedLine Citation:
PMID:  20223060     Owner:  NLM     Status:  MEDLINE    
Attenuated total reflection (ATR) Fourier transform infrared (FT-IR) spectroscopy has been applied to study the short and long term postmortem metabolic processes in rat and human kidney cortexes. The goals of this project were as follows: (1) to investigate the changes of ATR spectra in different rat and human tissues after death, (2) to explore the best mathematical model with different band absorption ratio changes to determine the postmortem interval (PMI), and (3) to establish a preliminary human postmortem ATR spectra database. There were three different types of metabolic changes after death based on the spectral results: (1) the intensities of some bands increased continuously (e.g., C-H stretching region), (2) the intensities of other bands decreased continuously (e.g., PO(2)(-) symmetric stretching), and (3) other bands remained relatively stable (e.g., C-OH bending, CO-O-C antisymmetric stretching). The band absorbance ratios for rats were found to display either a significant increase or decrease with increasing time after death. Of the absorbance ratios of the various bands investigated to find the best fit with the cubic model function in rats, the A(1652)/A(1396) ratio showed the strongest correlation (R(2) = 0.937). Comparison of the rat kidney cortex spectra with selected human postmortem cases showed similar postmortem metabolic changes. In conclusion, ATR FT-IR spectroscopy was shown to be a convenient and reliable method of determining short and long term postmortem intervals by simultaneously monitoring several specific parameters, although these observations have yet to be applied at forensic scenes by further field studies.
Ya Tuo; Ping Huang; Yong Ke; Shuanliang Fan; Qinyang Lu; Bo Xin; Zhenyuan Wang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied spectroscopy     Volume:  64     ISSN:  1943-3530     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-12     Completed Date:  2010-06-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  268-74     Citation Subset:  IM    
Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR China.
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MeSH Terms
Kidney Cortex / chemistry*,  metabolism
Models, Biological*
Models, Statistical
Pilot Projects
Postmortem Changes*
Rats, Sprague-Dawley
Spectroscopy, Fourier Transform Infrared / methods*

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