Document Detail

Attenuated purine production during subsequent ischemia in preconditioned rabbit myocardium is unrelated to the mechanism of protection.
MedLine Citation:
PMID:  9011628     Owner:  NLM     Status:  MEDLINE    
Preconditioned hearts release less purines during ischemia than virgin hearts. This study tested whether this reduced purine production is related to the mechanism of protection by ischemic preconditioning. Coronary effluent from isolated rabbit hearts was collected and purine (adenosine + inosine + hypoxanthine) levels were measured. All hearts underwent two cycles of 5 min global ischemia, each followed by 10 min reperfusion. In the first minute of reflow after the first ischemic period untreated hearts released 155 +/- 14 nmol purines per g wet weight, but only 104 +/- 16 nmol/g following the second bout of ischemia (P < 0.05). Thus, preconditioned hearts released less purines during ischemia. When 8-(p-sulfophenyl)theophylline (100 microM), which prevents the infarct size-limiting effect of ischemic preconditioning by blocking adenosine receptors was present in the perfusate, the pattern of purine release was not altered (151 +/- 13 nmol/g during the first minute after the first 5-min ischemic episode dropping to 117 +/- 6 nmol/g after the second ischemic period P < 0.05). Furthermore, pharmacological preconditioning with 5 min exposure of the heart to either adenosine (10 microM) or phenylephrine (0.1 microM) 15 min prior to the first ischemia did not affect purine release during early reperfusion after either the first (144 +/- 16 nmol/g and 153 +/- 12 nmol/g, respectively) or second (84 +/- 12 nmol/g and 111 +/- 9 nmol/g, respectively) bout of ischemia. Since the attenuated purine release was apparently unaffected by the presence or absence of a protected state, we conclude that this pattern is unrelated to the mechanism by which preconditioning protects the heart.
M Goto; M V Cohen; D G van Wylen; J M Downey
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  28     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1997-02-05     Completed Date:  1997-02-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  447-54     Citation Subset:  IM    
Department of Physiology, University of South Alabama, College of Medicine, Mobile 36688, USA.
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MeSH Terms
Adenosine / metabolism,  pharmacology
Heart / drug effects
Hypoxanthines / metabolism
Inosine / metabolism
Ischemic Preconditioning, Myocardial*
Myocardial Ischemia / metabolism*
Myocardium / metabolism*
Phenylephrine / pharmacology
Purines / biosynthesis*
Receptors, Purinergic P1 / antagonists & inhibitors,  metabolism
Theophylline / analogs & derivatives,  pharmacology
Grant Support
Reg. No./Substance:
0/Hypoxanthines; 0/Purines; 0/Receptors, Purinergic P1; 120-73-0/purine; 58-55-9/Theophylline; 58-61-7/Adenosine; 58-63-9/Inosine; 59-42-7/Phenylephrine; 80206-91-3/8-(4-sulfophenyl)theophylline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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