| Attenuated mitochondrial NADP(+)-dependent isocitrate dehydrogenase activity enhances EGCG-induced apoptosis. | |
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MedLine Citation:
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PMID: 21741430 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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(-)-Epigallocatechin-3-gallate (EGCG), a well-known chemopreventive factor, induces cancer cells undergoing apoptosis. Over the last several years, we have shown that the mitochondrial NADP(+)-dependent isocitrate dehydrogenase (IDPm) functions as an antioxidant and anti-apoptotic protein by supplying NADPH to antioxidant systems. Here, we show that EGCG induced the inactivation of IDPm as a purified enzyme and in cultured cancer cells in a dose- and time-dependent manner. Loss of enzyme activity was associated with the depletion of the thiol groups in protein. In addition, transfection of HeLa cells with an IDPm small interfering RNA (siRNA) markedly attenuated the activity of IDPm and substantially enhanced EGCG-induced apoptosis as indicated by the morphological evidence of apoptosis, DNA fragmentation, and the modulation of mitochondrial function and apoptotic marker proteins. Taken together, our results suggest that the suppression of IDPm activity resulted in the disruption of cellular redox balance and subsequently exacerbates EGCG-induced apoptotic cell death in HeLa cells. These results might have implications for developing an effective combination modality in cancer treatment. |
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Authors:
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In Sup Kil; Kyu Ho Jung; Woo Suk Nam; Jeen-Woo Park |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-2 |
Journal Detail:
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Title: Biochimie Volume: - ISSN: 1638-6183 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Masson SAS. |
Affiliation:
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School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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