| Attenuated expression of SECIS binding protein 2 causes loss of telomeric reserve without affecting telomerase. | |
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MedLine Citation:
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PMID: 19563878 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The family of selenoproteins have a broad range of functions, including protection against oxidative damage. Previous studies have shown that elevated levels of oxidative damage can induce accelerated loss of telomeric DNA during proliferation of mammalian cells. The incorporation of selenocysteine (Sec) into proteins in mammalian cells requires the Sec insertion sequence (SECIS) binding protein 2 (SBP2). Thus in the present study we have assessed the effect of knocking down the expression of SBP2 on telomere length. Following knock-down of SBP2 expression in two different human cell lines, the MSTO mesothelioma cell line ( approximately 5Kb average telomere length) and SY5Y neuroblastoma cell line (approximately 4.2Kb average telomere length), we observed a significant reduction (-0.6 to -1.1 Kb; P <or= 0.01) in telomere length as compared to control cells. This reduction in telomere length was independent of affects on telomerase, since both telomerase activity levels and Tert mRNA expression levels were not altered by knock-down of SBP2 expression. Furthermore, telomeres were particularly sensitive to S1 nuclease digestion following SBP2 knock-down, indicating an increased frequency of oxidative damage-induced lesions in the telomeric DNA in these cells. Together, these observations imply that selenoproteins may help protect telomeric reserve in mammalian cells. |
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Authors:
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Jeffery E Squires; Philip Davy; Marla J Berry; Rich Allsopp |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-06-27 |
Journal Detail:
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Title: Experimental gerontology Volume: 44 ISSN: 1873-6815 ISO Abbreviation: Exp. Gerontol. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0047061 Medline TA: Exp Gerontol Country: England |
Other Details:
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Languages: eng Pagination: 619-23 Citation Subset: IM |
Affiliation:
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Cell and Molecular Biology Department, John A Burns School of Medicine, Honolulu, HI 96813, USA. |
Export Citation:
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Descriptor/Qualifier:
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| Grant Support | |
ID/Acronym/Agency:
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DK47320/DK/NIDDK NIH HHS; R01 DK047320-10/DK/NIDDK NIH HHS |
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