| Attenuated cardiovascular hypertrophy and oxidant generation in response to angiotensin II infusion in glutaredoxin-1 knockout mice. | |
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MedLine Citation:
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PMID: 20638471 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glutaredoxin-1 (Glrx) is a thioltransferase that regulates protein S-glutathiolation. To elucidate the role of endogenous Glrx in cardiovascular disease, Glrx knockout (KO) mice were infused with angiotensin II (Ang II) for 6days. After Ang II infusion, body weight and blood pressure were similar between WT and Glrx KO mice. However, compared to WT mice, Glrx KO mice demonstrated (1) less cardiac and aortic medial hypertrophy, (2) less oxidant generation in aorta as assessed by dihydroethidium staining and nitrotyrosine, (3) decreased phosphorylation of Akt in the heart, and (4) less expression of inducible NOS in aorta and heart. In cultured embryonic fibroblasts from Glrx KO mice, S-glutathiolation of actin was enhanced and actin depolymerization was impaired after hydrogen peroxide stimulation compared with WT cells. Furthermore, oxidant generation in phorbol ester-stimulated fibroblasts and RAW 264.7 macrophage-like cells was lower with Glrx siRNA knockdown. These data indicate that Ang II-induced oxidant production and hypertrophic responses were attenuated in Glrx KO mice, which may result from impaired NADPH oxidase activation. |
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Authors:
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Markus M Bachschmid; Shanqin Xu; Karlene A Maitland-Toolan; Ye-Shih Ho; Richard A Cohen; Reiko Matsui |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-07-16 |
Journal Detail:
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Title: Free radical biology & medicine Volume: 49 ISSN: 1873-4596 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-08-30 Completed Date: 2011-04-11 Revised Date: 2011-10-17 |
Medline Journal Info:
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Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
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Languages: eng Pagination: 1221-9 Citation Subset: IM |
Copyright Information:
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Copyright 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Vascular Biology Unit, Department of Medicine, Boston University, Boston, MA 02118, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Angiotensin II
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administration & dosage Animals Aorta / pathology* Cardiovascular Diseases / genetics, metabolism, pathology, prevention & control* Cell Line Glutaredoxins / genetics, metabolism* Hypertrophy / genetics, metabolism, pathology, prevention & control* Infusion Pumps Mice Mice, Inbred C57BL Mice, Knockout Microfilaments / metabolism Myocardium / pathology* Nitric Oxide Synthase Type II / biosynthesis, genetics Oncogene Protein v-akt / genetics, metabolism Oxidants / metabolism RNA, Small Interfering / genetics Tyrosine / analogs & derivatives, metabolism |
| Grant Support | |
ID/Acronym/Agency:
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P01 HL081587/HL/NHLBI NIH HHS; P01 HL081587-030004/HL/NHLBI NIH HHS; P01 HL081587-040004/HL/NHLBI NIH HHS; P01 HL081587-050004/HL/NHLBI NIH HHS; R01 AG027080/AG/NIA NIH HHS; R01 AG027080-03/AG/NIA NIH HHS; R01 AG027080-04/AG/NIA NIH HHS; R01 AG027080-05/AG/NIA NIH HHS; R01 AG027080-05S1/AG/NIA NIH HHS; R01 HL104017/HL/NHLBI NIH HHS; R03 AG19078-01/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glrx protein, mouse; 0/Glutaredoxins; 0/Oxidants; 0/RNA, Small Interfering; 11128-99-7/Angiotensin II; 3604-79-3/3-nitrotyrosine; 55520-40-6/Tyrosine; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 2.7.11.1/Oncogene Protein v-akt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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