| Attenuated PGI2 synthesis in obese Zucker rats. | |
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MedLine Citation:
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PMID: 19118096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In obesity, skeletal muscle blood flow during exercise (functional hyperemia) is impaired. We have indirectly demonstrated that an altered arachidonic acid metabolism is responsible for the impaired functional vasodilation in the obese Zucker rat (OZR), a model of obesity. In this study, we tested the hypothesis that there is an impaired release of PGI(2) due to a nitration of PGI(2) synthase (PGIS), which is associated with a decreased prostanoid receptor expression. PGI(2), PGE(2), and thromboxane A(2) (TXA(2)) release were determined in vitro using ELISA under basal conditions and in response to arachidonic acid (AA) administration (50 microM). Immunofluorescence of PGI(2) and TXA(2) receptors (IP and TP, respectively) was determined in dispersed vascular smooth muscle cells (VSMCs). Nitration of tyrosine residues of the PGIS enzyme was determined using immunoprecipitation and Western blot analysis. Following AA administration, PGI(2) and PGE(2) release were attenuated in OZR compared with lean Zucker rats (LZR; controls). Basal and AA-induced TXA(2) release were not significantly different between groups. IP and TP immunofluorescence were not significantly different between OZR and LZR groups. OZR exhibited elevated nitration of tyrosine residues of PGIS compared with LZR. These results suggest that alterations in the PGI(2) pathway (attenuated PGI(2) synthesis), and not the TXA(2) pathway (normal TXA(2) synthesis/no change in TP receptor expression), underlie the attenuated functional hyperemia in the OZR. |
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Authors:
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Benjamin L Hodnett; Jennifer A Dearman; Cory B Carter; Robert L Hester |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-12-31 |
Journal Detail:
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Title: American journal of physiology. Regulatory, integrative and comparative physiology Volume: 296 ISSN: 0363-6119 ISO Abbreviation: Am. J. Physiol. Regul. Integr. Comp. Physiol. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-02-27 Completed Date: 2009-04-13 Revised Date: 2010-09-23 |
Medline Journal Info:
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Nlm Unique ID: 100901230 Medline TA: Am J Physiol Regul Integr Comp Physiol Country: United States |
Other Details:
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Languages: eng Pagination: R715-21 Citation Subset: IM |
Affiliation:
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Dept. of Physiology and Biophysics, Univ. of Mississippi Medical Center, Jackson, Mississippi 39216-4505, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid / metabolism Blotting, Western Cell Separation Epoprostenol / biosynthesis* Fluorescent Antibody Technique Male Microcirculation / physiology Myocytes, Smooth Muscle / metabolism Nitrates / metabolism Obesity / metabolism* Physical Conditioning, Animal / physiology Rats Rats, Zucker Receptors, Epoprostenol / biosynthesis Receptors, Thromboxane A2, Prostaglandin H2 / biosynthesis Tyrosine / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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HL-51971/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nitrates; 0/Receptors, Epoprostenol; 0/Receptors, Thromboxane A2, Prostaglandin H2; 35121-78-9/Epoprostenol; 506-32-1/Arachidonic Acid; 55520-40-6/Tyrosine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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