Document Detail


Attempts at producing a hybridised Penaeus mondon cell line by cellular fusion.
MedLine Citation:
PMID:  20580832     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The lack of a standardised system for the isolation, identification and purification of prawn viruses, is a major obstacle to the control of viruses in penaeid aquaculture. To date, spontaneous and induced transformation of somatic penaeid cells has failed. Hybrid cells with the aim of supporting the growth of penaeid viruses were created using polyethylene glycol (PEG)-mediated fusion with two immortal cell lines, Epithelioma papulosum cyprinid (EPC) and Spodoptera frugiperda pupal ovarian cells (Sf9), fused with Penaeus monodon haemocytes. The immortal cell lines were biochemically blocked with actinomycin D and puromycin before fusion occurred. A total of 78 hybrid clones were created. The methods used to confirm the presence of P. monodon genes and proteins in the hybrid cells did not detect crustacean components, nor was any viral amplification detected by real-time PCR after hybrid cells were inoculated with two P. monodon parvoviruses, Penaeus merguiensis densovirus and infectious hypodermal and haematopoietic necrosis virus. These results suggest although the creation of the hybrid cells appeared successful, the cell lines lacked key crustacean cell components required for their use as an in vitro system for virus replication.
Authors:
Kerry Claydon; Katrina G Roper; Leigh Owens
Publication Detail:
Type:  Journal Article     Date:  2010-05-24
Journal Detail:
Title:  Fish & shellfish immunology     Volume:  29     ISSN:  1095-9947     ISO Abbreviation:  Fish Shellfish Immunol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-12     Completed Date:  2010-10-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9505220     Medline TA:  Fish Shellfish Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  539-43     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
Affiliation:
School of Veterinary and Biomedical Sciences, James Cook University, Queensland 4811, Australia. kerryclaydon@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Fusion
Cell Line
Hemocytes / cytology
Hybrid Cells / cytology
Penaeidae / cytology*

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