Document Detail


Atrial natriuretic peptide stability.
MedLine Citation:
PMID:  18539145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Atrial natriuretic peptide (ANP) is a key regulator in the homeostasis of water excretion and has emerged as an important prognostic marker for symptomatic chronic heart failure (CHF). The stability of ANP represents a crucial factor in assessing its use as a cardiac biomarker. Accordingly, we assessed the stability of ANP in blood samples collected from healthy controls and CHF subjects for a 12 month period. METHODS: Blood samples from 10 healthy controls and 12 symptomatic CHF subjects with left ventricular systolic dysfunction were drawn. Determination of plasma ANP was performed by a standardized radioimmunoassay protocol. RESULTS: The ANP levels of healthy subjects were 68.5+/-11.6 pg/mL at baseline and 69.9+/-17.2 pg/mL at 12 months (p=0.71). The ANP concentrations of CHF subjects were 199.25+/-44.8 pg/mL at baseline and 197.83+/-47.4 pg/mL at 12 months (p=0.70) respectively. CONCLUSION: ANP is a stable molecule with no evidence of degradation when stored at -80 degrees C.
Authors:
Ronald Zolty; Carol Bauer; Pamila Allen; Maureen Garrity; Timothy J Vittorio
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Publication Detail:
Type:  Journal Article     Date:  2008-05-27
Journal Detail:
Title:  Clinical biochemistry     Volume:  41     ISSN:  1873-2933     ISO Abbreviation:  Clin. Biochem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-20     Completed Date:  2008-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0133660     Medline TA:  Clin Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1255-8     Citation Subset:  IM    
Affiliation:
Albert Einstein College of Medicine, Division of Cardiology, Bronx, NY 10461, USA. RZOLTY@montefiore.org
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MeSH Terms
Descriptor/Qualifier:
Adult
Atrial Natriuretic Factor / blood*
Case-Control Studies
Chronic Disease
Female
Heart Failure / blood
Humans
Male
Middle Aged
Protein Stability
Thermodynamics
Chemical
Reg. No./Substance:
85637-73-6/Atrial Natriuretic Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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