| Atrial natriuretic factor stimulates phosphorylation of a 52-kDa calmodulin-binding protein in vascular smooth muscle cells. | |
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MedLine Citation:
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PMID: 7681659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effect of atrial natriuretic factor (ANF) on the phosphorylation of the calmodulin-binding protein in vascular smooth muscle cells was investigated. Several phosphorylated calmodulin-binding proteins ranging in molecular weight from 205,000 to 50,000 were detected. Among them, we have found that the phosphorylation of a 52-kDa protein present mainly in the cytosolic fraction is stimulated by ANF and that the elevation of the phosphorylation is both time- and dose-dependent. Furthermore, the stimulation was mimicked by 8-bromo-cyclic GMP but not by 8-bromo-cyclic AMP. Endothelin induced significant inhibition of the phosphorylation. These results indicate that 52-kDa protein phosphorylation may be responsible for the regulation of vascular smooth muscle tone. |
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Authors:
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S Nakajo; T Inagami |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Archives of biochemistry and biophysics Volume: 301 ISSN: 0003-9861 ISO Abbreviation: Arch. Biochem. Biophys. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-23 Completed Date: 1993-04-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372430 Medline TA: Arch Biochem Biophys Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 320-4 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / cytology, metabolism* Atrial Natriuretic Factor / pharmacology* Calmodulin-Binding Proteins / metabolism* Cells, Cultured Cyclic GMP / analogs & derivatives, pharmacology Cytosol / metabolism Dose-Response Relationship, Drug Endothelins / pharmacology Male Muscle, Smooth, Vascular / drug effects, metabolism* Phosphorylation Phosphoserine / analysis Phosphothreonine / analysis Phosphotyrosine Protein Kinases / metabolism* Rats Rats, Inbred Strains Rats, Wistar Tyrosine / analogs & derivatives, analysis |
| Grant Support | |
ID/Acronym/Agency:
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HL14192/HL/NHLBI NIH HHS; HL35323/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin-Binding Proteins; 0/Endothelins; 1114-81-4/Phosphothreonine; 17885-08-4/Phosphoserine; 21820-51-9/Phosphotyrosine; 31356-94-2/8-bromocyclic GMP; 55520-40-6/Tyrosine; 7665-99-8/Cyclic GMP; 85637-73-6/Atrial Natriuretic Factor; EC 2.7.-/Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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