Document Detail

Atrial natriuretic factor receptors in cultured renomedullary interstitial cells.
MedLine Citation:
PMID:  2159215     Owner:  NLM     Status:  MEDLINE    
To gain further insight on the cell types that may mediate the effects of atrial natriuretic factor (ANF) in the renal medulla, we determined the distribution and function of biological (B) and clearance (C) receptors of ANF in renomedullary interstitial cells (RMIC). Studies were performed in the 3rd-17th passages of RMIC obtained from a primary culture of the rat renal medulla. Electron microscopy of the cultured cells showed the typical morphological features of RMIC "in vivo," including prominent lipid droplets. RMIC have a very high density of high-affinity specific binding sites of ANF-(1-28) [23,000 sites/cell; dissociation constant (Kd) = 50 pM]. There was only minimal binding of C-ANF-(4-23) (less than 2,500 sites/cell), a specific ligand of C-ANF receptors. ANF-(1-28) markedly increased guanosine 3',5'-cyclic monophosphate (cGMP) from 1.3 +/- 0.3 to 106 +/- 22 pmol cGMP/10(6) cells [50% effective dosage (ED50) = 1.2 nM]. The effect of ANF-(1-28) on cGMP was nearly additive to that of sodium nitroprusside and was not potentiated or antagonized by C-ANF-(4-23). The density of guanylate cyclase-coupled B-ANF receptors and the ANF-induced increase in cGMP in RMIC are higher than those reported to date in other target cells. This suggests that RMIC may mediate some of the known effects of ANF in the renal medulla.
B M Fontoura; D R Nussenzveig; K M Pelton; T Maack
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  258     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-06-05     Completed Date:  1990-06-05     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C692-9     Citation Subset:  IM    
Department of Physiology, Cornell University Medical College, New York, New York 10021.
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MeSH Terms
Atrial Natriuretic Factor / metabolism*
Binding, Competitive
Cells, Cultured
Chromatography, High Pressure Liquid
Kidney Medulla / cytology,  metabolism*,  ultrastructure
Microscopy, Electron
Rats, Inbred Strains
Receptors, Atrial Natriuretic Factor
Receptors, Cell Surface / isolation & purification,  metabolism*
Grant Support
Reg. No./Substance:
0/Receptors, Cell Surface; 85637-73-6/Atrial Natriuretic Factor; EC, Atrial Natriuretic Factor

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