Document Detail


Atrial natriuretic factor inhibits the phosphorylation of protein kinase C in plasma membrane preparations of cultured Leydig tumor cells.
MedLine Citation:
PMID:  8056632     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The peptide hormone atrial natriuretic factor (ANF) exerts its effect in a receptor-mediated fashion and the membrane-bound form of guanylate cyclase represents a biologically active ANF receptor; thus, cGMP has been considered a second messenger of ANF. To understand the mechanisms of ANF action, we have studied its effect on protein phosphorylation in the plasma membrane preparations of murine Leydig tumor (MA-10) cells, which overexpress guanylate cyclase-coupled ANF receptor molecules in high density. After pretreatment of the plasma membranes with ANF (100 nM), a marked decrease in phosphorylation of the 78-kDa protein kinase C (PKC) and the 240-kDa protein was observed. Phosphorylation of the 78-kDa PKC was also inhibited by cGMP (0.1 mM); however, phosphorylation of the 240-kDa protein was not affected by cGMP. The quantitative analyses, as determined by densitometric scanning, revealed that both ANF and cGMP inhibited phosphorylation of the 78-kDa PKC by approximately 75% and 45%, respectively. The inhibitory effect of ANF on phosphorylation of the 240-kDa protein was almost 90%, but cGMP did not show any discernible effect on its phosphorylation in plasma membranes of MA-10 cells. Phosphorylation of the 78-kDa PKC was stimulated by Ca2+ and phospholipids, and it immunologically cross-reacted with antiserum against brain PKC. Furthermore, in these plasma membrane preparations, the 78-kDa PKC was immunoprecipitated and its phosphorylation was inhibited by ANF. These data provide evidence for a new signal transduction mechanism of ANF that negatively regulates phosphorylation of the 78-kDa PKC and the 240-kDa protein in a cGMP-dependent and -independent manner in Leydig cells.
Authors:
K N Pandey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of andrology     Volume:  15     ISSN:  0196-3635     ISO Abbreviation:  J. Androl.     Publication Date:    1994 Mar-Apr
Date Detail:
Created Date:  1994-09-12     Completed Date:  1994-09-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8106453     Medline TA:  J Androl     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  100-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Medical College of Georgia, School of Medicine, Augusta 30912.
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MeSH Terms
Descriptor/Qualifier:
Animals
Atrial Natriuretic Factor / physiology*
Cell Membrane / enzymology
Leydig Cell Tumor
Leydig Cells / enzymology,  metabolism*
Male
Membrane Proteins / metabolism
Mice
Phosphorylation
Protein Kinase C / antagonists & inhibitors*
Tumor Cells, Cultured
Grant Support
ID/Acronym/Agency:
HD 25527/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Membrane Proteins; 85637-73-6/Atrial Natriuretic Factor; EC 2.7.11.13/Protein Kinase C

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