| Atresia in follicles grown after ovulation in the pig: measurement of increased apoptosis in granulosa cells and reduced follicular fluid estradiol-17 beta. | |
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MedLine Citation:
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PMID: 7780014 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The incidence of atresia in the first group of follicles grown after ovulation was investigated in the pig. At slaughter, 113 follicles 3-6 mm in diameter were dissected from the ovaries of four pregnant pigs per day on Days 5, 6, and 7 after the onset of estrus. Granulosa cells were isolated from each follicle. The percentage of granulosa cells containing sub-diploid amounts of DNA (%Ao cells), a measure of apoptosis, was determined for each follicle by DNA fluorescence flow cytometry of propidium iodide (PI) stained nuclei of ethanol-fixed cells. Granulosa cell DNA condition was used to classify follicles. Follicles with > or = 10% Ao cells (n = 33) were designated biochemically atretic (BA), and follicles with < 10% Ao cells (n = 80) were designated biochemically healthy (BH). Internucleosomal cleavage, also indicative of apoptosis, was determined by autoradiographic analysis of [32P]-3'-end-labeled DNA from granulosa cells. Densitometric analysis showed that optical density of [32P]-3'-end-labeled DNA fragments in the 0.18-20-kb size range was correlated with the %Ao cells (R > or = 0.90, N = 22, p < 0.001). The incidence of pigs with BA follicles was 2 of 4, 3 of 4, and 4 of 4 on Days 5, 6, and 7, respectively. The %BA follicles per pig (mean +/- SEM) increased (p < or = 0.01) between Days 5 and 7; values were 6.2 +/- 3.6, 28.1 +/- 13.5, and 50.0 +/- 7.1, respectively, on Days 5, 6, and 7.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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H D Guthrie; B S Cooper; G R Welch; A D Zakaria; L A Johnson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biology of reproduction Volume: 52 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1995 Apr |
Date Detail:
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Created Date: 1995-07-20 Completed Date: 1995-07-20 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 920-7 Citation Subset: IM |
Affiliation:
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Germplasm and Gamete Physiology Laboratory, U.S. Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis* Autoradiography Cell Cycle DNA / analysis, metabolism Estradiol / metabolism* Female Flow Cytometry Follicular Atresia / physiology* Follicular Fluid / metabolism* Granulosa Cells / chemistry, physiology* Ovulation / physiology* Swine |
| Chemical | |
Reg. No./Substance:
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50-28-2/Estradiol; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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