Document Detail


Atresia in follicles grown after ovulation in the pig: measurement of increased apoptosis in granulosa cells and reduced follicular fluid estradiol-17 beta.
MedLine Citation:
PMID:  7780014     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The incidence of atresia in the first group of follicles grown after ovulation was investigated in the pig. At slaughter, 113 follicles 3-6 mm in diameter were dissected from the ovaries of four pregnant pigs per day on Days 5, 6, and 7 after the onset of estrus. Granulosa cells were isolated from each follicle. The percentage of granulosa cells containing sub-diploid amounts of DNA (%Ao cells), a measure of apoptosis, was determined for each follicle by DNA fluorescence flow cytometry of propidium iodide (PI) stained nuclei of ethanol-fixed cells. Granulosa cell DNA condition was used to classify follicles. Follicles with > or = 10% Ao cells (n = 33) were designated biochemically atretic (BA), and follicles with < 10% Ao cells (n = 80) were designated biochemically healthy (BH). Internucleosomal cleavage, also indicative of apoptosis, was determined by autoradiographic analysis of [32P]-3'-end-labeled DNA from granulosa cells. Densitometric analysis showed that optical density of [32P]-3'-end-labeled DNA fragments in the 0.18-20-kb size range was correlated with the %Ao cells (R > or = 0.90, N = 22, p < 0.001). The incidence of pigs with BA follicles was 2 of 4, 3 of 4, and 4 of 4 on Days 5, 6, and 7, respectively. The %BA follicles per pig (mean +/- SEM) increased (p < or = 0.01) between Days 5 and 7; values were 6.2 +/- 3.6, 28.1 +/- 13.5, and 50.0 +/- 7.1, respectively, on Days 5, 6, and 7.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H D Guthrie; B S Cooper; G R Welch; A D Zakaria; L A Johnson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biology of reproduction     Volume:  52     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1995 Apr 
Date Detail:
Created Date:  1995-07-20     Completed Date:  1995-07-20     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  920-7     Citation Subset:  IM    
Affiliation:
Germplasm and Gamete Physiology Laboratory, U.S. Department of Agriculture Agricultural Research Service, Beltsville, Maryland 20705, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis*
Autoradiography
Cell Cycle
DNA / analysis,  metabolism
Estradiol / metabolism*
Female
Flow Cytometry
Follicular Atresia / physiology*
Follicular Fluid / metabolism*
Granulosa Cells / chemistry,  physiology*
Ovulation / physiology*
Swine
Chemical
Reg. No./Substance:
50-28-2/Estradiol; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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