Document Detail


Atrazine degradation by encapsulated Rhodococcus erythropolis NI86/21.
MedLine Citation:
PMID:  16162227     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: To develop an encapsulation procedure for Rhodococcus erythropolis NI86/21 and demonstrate its use as a slow-release inoculant for reducing atrazine levels in aquatic and terrestrial environments. METHODS AND RESULTS: Alginate encapsulation procedures were developed for the atrazine-degrading bacteria R. erythropolis NI86/21. Several bead amendments, including bentonite, powdered activated carbon (PAC) and skimmed milk (SM), were evaluated for slow release of R. erythropolis NI86/21 and efficacy of atrazine degradation. All bead types demonstrated a capacity to degrade atrazine in basal minimal nutrient buffer whilst continually releasing viable bacterial cells. We found that the addition of bentonite hastened cell release whilst SM sustained cell viability in bead formulations. Reducing the percentage of SM to 1% (w/v) resulted in faster rates of atrazine degradation in both liquid and soil, and was found to prolong cell survival upon bead storage. Limited oxygen transfer affects the capacity of the encapsulated R. erythropolis cells to degrade atrazine. CONCLUSIONS: Degradation studies have demonstrated the efficacy of R. erythropolis encapsulated cells to degrade atrazine in amended liquid and soil. However, in their current formulation, the wet alginate-based beads are impractical for field application because of their poor cell viability during storage. SIGNIFICANCE AND IMPACT OF THE STUDY: R. erythropolis NI86/21-encapsulated cells have the potential to reduce atrazine residues in a number of soil and water environments, possibly ensuring the continued registration and use of atrazine in agriculture by minimizing or eliminating nontarget effects.
Authors:
T Vancov; K Jury; L Van Zwieten
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  99     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-09-15     Completed Date:  2006-02-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  767-75     Citation Subset:  IM    
Affiliation:
New South Wales Department of Primary Industries, Wollongbar Agricultural Institute, Wollongbar, NSW, Australia. tony.vancov@dpi.nsw.gov.au
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MeSH Terms
Descriptor/Qualifier:
Alginates / metabolism
Atrazine / adverse effects,  metabolism*
Delayed-Action Preparations / metabolism
Herbicides / adverse effects,  metabolism*
Microspheres
Rhodococcus / metabolism*
Soil Microbiology
Soil Pollutants / adverse effects
Water Microbiology
Water Pollutants, Chemical / adverse effects
Chemical
Reg. No./Substance:
0/Alginates; 0/Delayed-Action Preparations; 0/Herbicides; 0/Soil Pollutants; 0/Water Pollutants, Chemical; 1912-24-9/Atrazine

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