| Atractylenolide II induces G1 cell-cycle arrest and apoptosis in B16 melanoma cells. | |
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MedLine Citation:
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PMID: 21524699 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIM OF THE STUDY: Atractylenolide II (AT-II) is a sesquiterpene compound isolated from the dried rhizome of Atractylodes macrocephala (Baizhu in Chinese), which is traditionally prescribed for melanoma treatment by Chinese medicine practitioners. Our previous study showed that AT-II can inhibit B16 cells proliferation. Here we investigated the mechanistic basis for the anti-proliferative activity of AT-II in B16 melanoma cells. MATERIALS AND METHODS: Cell viability was examined by MTT assay. Cell cycle distribution and apoptosis were determined by flow cytometry. Protein expression was determined by Western blotting. RESULTS: AT-II treatment for 48h dose-dependently inhibited cell proliferation with an IC(50) of 82.3μM, and induced G1 phase cell cycle arrest. Moreover, treatment with 75μM AT-II induced apoptosis. These observations were associated with the decrease of the expression of Cdk2, phosphorylated-Akt, phosphorylated-ERK and Bcl-2, the increase of the expression of phosphorylated-p38, phosphorylated-p53, p21, p27, and activation of caspases-8, -9 and -3. In addition, a chemical inhibitor of p53, PFTα, significantly decreased AT-II-mediated growth inhibition and apoptosis. CONCLUSIONS: We demonstrated that the G1-arresting and apoptotic effects of AT-II in B16 cells involve p38 activation and ERK inactivation, and the cytotoxic/apoptotic effects of AT-II are at least in part mediated by PI3K/Akt signaling and potentially p53 dependent. These findings provided chemical and pharmacological basis for the traditional application of Baizhu in melanoma treatment. |
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Authors:
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Yan Ye; Hui Wang; Jian-Hong Chu; Gui-Xin Chou; Si-Bao Chen; Huanbiao Mo; Wang-Fun Fong; Zhi-Ling Yu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-4-16 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: - ISSN: 1872-7573 ISO Abbreviation: - Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-4-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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