Document Detail


Atonal and EGFR signalling orchestrate rok- and Drak-dependent adherens junction remodelling during ommatidia morphogenesis.
MedLine Citation:
PMID:  22874916     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Morphogenesis of epithelial tissues relies on the interplay between cell division, differentiation and regulated changes in cell shape, intercalation and sorting. These processes are often studied individually in relatively simple epithelia that lack the complexity found during organogenesis when these processes might all coexist simultaneously. To address this issue, we are making use of the developing fly retinal neuroepithelium. Retinal morphogenesis relies on a coordinated sequence of interdependent morphogenetic events that includes apical cell constriction, localized alignment of groups of cells and ommatidia morphogenesis coupled to neurogenesis. Here, we use live imaging to document the sequence of adherens junction (AJ) remodelling events required to generate the fly ommatidium. In this context, we demonstrate that the kinases Rok and Drak function redundantly during Myosin II-dependent cell constriction, subsequent multicellular alignment and AJ remodelling. In addition, we show that early multicellular patterning characterized by cell alignment is promoted by the conserved transcription factor Atonal (Ato). Further ommatidium patterning requires the epidermal growth factor receptor (EGFR) signalling pathway, which transcriptionally governs rok- and Drak-dependent AJ remodelling while also promoting neurogenesis. In conclusion, our work reveals an important role for Drak in regulating AJ remodelling during retinal morphogenesis. It also sheds new light on the interplay between Ato, EGFR-dependent transcription and AJ remodelling in a system in which neurogenesis is coupled with cell shape changes and regulated steps of cell intercalation.
Authors:
Francesca Robertson; Noelia Pinal; Pierre Fichelson; Franck Pichaud
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-08-08
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  139     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-22     Completed Date:  2012-10-29     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3432-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adherens Junctions / metabolism*
Animals
Basic Helix-Loop-Helix Transcription Factors / genetics,  metabolism*
Drosophila
Drosophila Proteins / genetics,  metabolism*
Morphogenesis / genetics,  physiology*
Nerve Tissue Proteins / genetics,  metabolism*
Protein-Serine-Threonine Kinases / genetics,  metabolism*
Receptor, Epidermal Growth Factor / genetics,  metabolism*
Signal Transduction / genetics,  physiology
rho-Associated Kinases / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
MC-US-A710//Medical Research Council; MC_U122673973//Medical Research Council
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Drosophila Proteins; 0/Nerve Tissue Proteins; 0/ato protein, Drosophila; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.11.1/Drak protein, Drosophila; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/rho-Associated Kinases
Comments/Corrections

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