| Atomic force microscopy to study direct neurite-mast cell (RBL) communication in vitro. | |
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MedLine Citation:
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PMID: 11064103 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. We used an in vitro co-culture approach comprising cultured murine superior cervical ganglia (SCG) and rat basophilic leukemia (RBL-2H3) cells. Atomic force microscopy (AFM) showed how neurites attached to a pseudopodium or a cell body of an RBL cell. After stimulation of SCG neurites with bradykinin or scorpion venom, RBL cells attached to neurites spread and flattened, and several discharged granules (0. 5-1.0 microm in diameter) were found on the surface of the RBL cells. A neurokinin (NK)-1 receptor (i.e. substance P receptor) antagonist prevented the RBL degranulation. The results showed that activation of the SCG neurites with bradykinin or scorpion venom was able to elicit degranulation in RBL cells which were attached to neurites. |
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Authors:
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H Ohshiro; R Suzuki; T Furuno; M Nakanishi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Immunology letters Volume: 74 ISSN: 0165-2478 ISO Abbreviation: Immunol. Lett. Publication Date: 2000 Nov |
Date Detail:
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Created Date: 2001-01-04 Completed Date: 2001-01-04 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7910006 Medline TA: Immunol Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 211-4 Citation Subset: IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, 467-8603, Nagoya, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Basophil Degranulation Test Bradykinin / pharmacology Calcium Signaling / drug effects Cell Communication* Cells, Cultured / drug effects Cytoplasmic Granules / secretion Leukemia, Basophilic, Acute / pathology Mast Cells / physiology*, secretion, ultrastructure Mice Mice, Inbred CBA Microscopy, Atomic Force* Neurites / drug effects, physiology*, ultrastructure Pseudopodia / physiology*, ultrastructure Rats Scorpion Venoms / pharmacology Superior Cervical Ganglion / cytology, drug effects Tumor Cells, Cultured / physiology, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Scorpion Venoms; 58-82-2/Bradykinin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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