Document Detail

Atomic force microscopy to study direct neurite-mast cell (RBL) communication in vitro.
MedLine Citation:
PMID:  11064103     Owner:  NLM     Status:  MEDLINE    
Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. We used an in vitro co-culture approach comprising cultured murine superior cervical ganglia (SCG) and rat basophilic leukemia (RBL-2H3) cells. Atomic force microscopy (AFM) showed how neurites attached to a pseudopodium or a cell body of an RBL cell. After stimulation of SCG neurites with bradykinin or scorpion venom, RBL cells attached to neurites spread and flattened, and several discharged granules (0. 5-1.0 microm in diameter) were found on the surface of the RBL cells. A neurokinin (NK)-1 receptor (i.e. substance P receptor) antagonist prevented the RBL degranulation. The results showed that activation of the SCG neurites with bradykinin or scorpion venom was able to elicit degranulation in RBL cells which were attached to neurites.
H Ohshiro; R Suzuki; T Furuno; M Nakanishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunology letters     Volume:  74     ISSN:  0165-2478     ISO Abbreviation:  Immunol. Lett.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-01-04     Completed Date:  2001-01-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7910006     Medline TA:  Immunol Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  211-4     Citation Subset:  IM    
Faculty of Pharmaceutical Sciences, Nagoya City University, Tanabe-dori, Mizuho-ku, 467-8603, Nagoya, Japan.
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MeSH Terms
Basophil Degranulation Test
Bradykinin / pharmacology
Calcium Signaling / drug effects
Cell Communication*
Cells, Cultured / drug effects
Cytoplasmic Granules / secretion
Leukemia, Basophilic, Acute / pathology
Mast Cells / physiology*,  secretion,  ultrastructure
Mice, Inbred CBA
Microscopy, Atomic Force*
Neurites / drug effects,  physiology*,  ultrastructure
Pseudopodia / physiology*,  ultrastructure
Scorpion Venoms / pharmacology
Superior Cervical Ganglion / cytology,  drug effects
Tumor Cells, Cultured / physiology,  ultrastructure
Reg. No./Substance:
0/Scorpion Venoms; 58-82-2/Bradykinin

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