Document Detail

Atomic force microscopy study of different effects of natural and semisynthetic beta-lactam on the cell envelope of Escherichia coli.
MedLine Citation:
PMID:  17037942     Owner:  NLM     Status:  MEDLINE    
The effects of native and semisynthetic beta-lactam on the surface of E. coli cells were investigated using tapping mode atomic force microscopy (AFM). These studies were done on both single cell and cell colonies. This allowed us to achieve a complete understanding of the effects, since previous related AFM analysis was only performed on single cells. Our results indicated that although amoxycillin and penicillin could both induce nanoporous damage to the envelope of E. coli, the distribution of the pores was different: those induced by penicillin were randomly distributed on the cell surface, while those induced by amoxycillin were far more numerous and mainly on the two ends of the cell. These findings could explain why the effect of amoxycillin is stronger than that of native penicillin. Ofloxacin was used as a control due to its inactive membrane, and no cell wall damages were observed. Our results demonstrate that AFM is a useful tool in discerning and verifying antibiotic mechanisms and can be helpful to explain the relationship between chemical structure and the function of antibiotics.
Liu Yang; Kemin Wang; Weihong Tan; Xiaoxiao He; Rong Jin; Jun Li; Huimin Li
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  78     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-10-13     Completed Date:  2007-04-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7341-5     Citation Subset:  IM    
State Key Laboratory of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Institution of Life Science and Technology, Hunan University, Changsha 410082, PR China.
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MeSH Terms
Anti-Bacterial Agents / chemistry,  pharmacology
Biological Products / chemistry,  pharmacology*
Cell Membrane / drug effects*,  ultrastructure*
Escherichia coli / cytology,  drug effects*,  ultrastructure
Microscopy, Atomic Force / methods*
Molecular Structure
Time Factors
beta-Lactams / chemical synthesis,  chemistry*,  pharmacology*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Biological Products; 0/beta-Lactams

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