Document Detail

Atomic force microscopy of microvillous cell surface dynamics at fixed and living alveolar type II cells.
MedLine Citation:
PMID:  21116619     Owner:  NLM     Status:  In-Data-Review    
Scanning probe techniques enable direct imaging of morphology changes associated with cellular processes at life specimen. Here, glutaraldehyde-fixed and living alveolar type II (ATII) cells were investigated by atomic force microscopy (AFM), and the obtained topographical data were correlated with results obtained by scanning electron microscopy (SEM) and confocal microscopy (CM). We show that low-force contact mode AFM at glutaraldehyde-fixed cells provides complementary results to SEM and CM. Both AFM and SEM images reveal fine structures at the surface of fixed cells, which indicate microvilli protrusions. If ATII cells were treated with Ca(2+) channel modulators known to induce massive endocytosis, changes of the cell surface topography became evident by the depletion of microvilli. Low force contact mode AFM imaging at fixed ATII cells revealed a significant reduction of the surface roughness for capsazepine and 2-aminoethoxydiphenyl-borate (CPZ/2-APB)-treated cells compared to untreated control cells (Rc of 99.7 ± 6.8 nm vs. Rc of 71.9 ± 4.6 nm for N = 22), which was confirmed via SEM studies. CM of microvilli marker protein Ezrin revealed a cytoplasmic localization of Ezrin in CPZ/2-APB-treated cells, whereas a submembranous Ezrin localization was observed in control cells. Furthermore, in situ AFM investigations at living ATII cells using low force contact mode imaging revealed an apparent decrease in cell height of 17% during stimulation experiments. We conclude that a dynamic reorganization of the microvillous cell surface occurs in ATII cells at conditions of stimulated endocytosis.
Elena Hecht; Shariq M Usmani; Susanne Albrecht; Oliver H Wittekindt; Paul Dietl; Boris Mizaikoff; Christine Kranz
Related Documents :
20726809 - Microparticulate polyelectrolyte complexes for gentamicin transport across intestinal e...
14739529 - Paradigms for mechanical signal transduction in the intestinal epithelium. category: mo...
15116099 - Hst-1/fgf-4 plays a critical role in crypt cell survival and facilitates epithelial cel...
10510049 - Early in vivo interactions of actinobacillus pleuropneumoniae with tonsils of pigs.
617769 - Influence of the intestinal epithelium to the plasma cell differentiation injected into...
3315649 - Characterization of the cell adhesion molecules l1, n-cam and j1 in the mouse intestine.
19916819 - Adipose-derived stem cell delivery into collagen gels using chitosan microspheres.
8985319 - From essential to beneficial: glycoprotein d loses importance for replication of bovine...
24623559 - Cell surface antigen profiling using a novel type of antibody array immobilised to plas...
Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  399     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2369-78     Citation Subset:  IM    
Institute of Analytical and Bioanalytical Chemistry, University of Ulm, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Study of the gas chromatographic behavior of selected alcohols and amines.
Next Document:  Validated hydrophilic interaction LC-MS/MS method for determination of 2-pyrrolidinone residue: appl...