Document Detail


Atomic force microscopy functional imaging on vascular endothelial cells.
MedLine Citation:
PMID:  23027010     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy high-resolution receptor mapping. In this chapter, we provide a flavor of TREC application on vascular endothelial cells by describing the detailed procedures for all stages of the experiment from tip and sample preparations through the operating principles and visualization.
Authors:
Lilia A Chtcheglova; Peter Hinterdorfer
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  931     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-02     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  331-44     Citation Subset:  IM    
Affiliation:
Center for Advanced Bioanalysis (CBL GmbH), Linz, Austria, lilia.chtcheglova@cbl.at.
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