| Atomic force microscopy functional imaging on vascular endothelial cells. | |
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MedLine Citation:
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PMID: 23027010 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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One of the challenging tasks in molecular cell biology is to identify and localize specific binding sites on biological samples with high spatial accuracy (in order of several nm). During the past 5 years, simultaneous topography and recognition imaging (TREC) has become a powerful AFM-based technique for quick and easy high-resolution receptor mapping. In this chapter, we provide a flavor of TREC application on vascular endothelial cells by describing the detailed procedures for all stages of the experiment from tip and sample preparations through the operating principles and visualization. |
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Authors:
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Lilia A Chtcheglova; Peter Hinterdorfer |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 931 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-10-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 331-44 Citation Subset: IM |
Affiliation:
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Center for Advanced Bioanalysis (CBL GmbH), Linz, Austria, lilia.chtcheglova@cbl.at. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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