| Atomic Resolution Structure of an N(5) Flavin Adduct in D-Arginine Dehydrogenase. | |
| | |
MedLine Citation:
|
PMID: 21707047 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
D-Arginine dehydrogenase (DADH) catalyzes the flavin-dependent oxidative deamination of D-arginine and other D-amino acids to the corresponding imino acids. The 1.07 Å atomic resolution structure of DADH crystallized with D-leucine unexpectedly revealed a covalent flavin N(5) adduct, instead of the expected iminoleucine product in the active site. This acyl adduct has been successfully reproduced by photoreduction of DADH in the presence of 4-methyl-2-oxopentanoic acid (ketoleucine). The iminoleucine may be released readily due to weak interactions in the binding site, in contrast to iminoarginine, converted to ketoleucine, which reacts with activated FAD to form the covalently linked acyl adduct. |
| | |
Authors:
|
Guoxing Fu; Hongling Yuan; Siming Wang; Giovanni Gadda; Irene T Weber |
Related Documents
:
|
21600977 - Radiosensitizing effect of ferulic acid on human cervical carcinoma cells in vitro. 20579527 - The phenolic acids from bacterial degradation of the mangiferin aglycone are quantified... 21617997 - Determination of total antioxidant capacity by a new spectrofluorometric method based o... 21753077 - Soluble epoxide hydrolase contamination of specific catalase preparations inhibits epox... 6777867 - Effect of hyaluronic acid on phagocytosis of opsonized latex particles. 6292417 - Hydroxy- and amino-substituted piperidinecarboxylic acids as gamma-aminobutyric acid ag... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-6-27 |
Journal Detail:
|
Title: Biochemistry Volume: - ISSN: 1520-4995 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
|
Created Date: 2011-6-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Isolation and Biological Evaluation of Jatrophane Diterpenoids from Euphorbia dendroides.
Next Document: Turn-on Aptameric System for Simple and Selective Detection of Protein via Base Stacking-dependent D...