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Atomic Force Microscopy Study of Ganglioside GM1 Concentration Effect on Lateral Phase Separation of Sphingomyelin/Dioleoylphosphatidylcholine/Cholesterol Bilayers.
MedLine Citation:
PMID:  21526782     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The effect of monosialoganglioside GM1 (GM1) concentration on the lateral phase separation in the sphingomyelin/1,2-dioleoyl-sn-glycero-3-phosphocholine/cholesterol (SM/DOPC/Chol) bilayers was studied by using atomic force microscopy. The results show that, with the increase of GM1 mol fraction (x), the dominant composition of liquid-ordered (L(o)) domains changes from SM to SM/GM1 and finally to GM1. Meanwhile, the decrease of domain area (A) of the L(o) phase with the increase of x follows a scaling law of A ∼ x(-3/2), for x > 0.005, indicating that the domain growth is pinned with high GM1 concentration. Results of in situ experiments of GM1 insertion into SM/DOPC/cholesterol bilayers further supported our observations.
Authors:
Ren Bao; Li Li; Feng Qiu; Yuliang Yang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-28
Journal Detail:
Title:  The journal of physical chemistry. B     Volume:  -     ISSN:  1520-5207     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-4-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101157530     Medline TA:  J Phys Chem B     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
The Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, Department of Macromolecular Science, the Centre of Analysis and Measurement, Fudan University , Shanghai 200433, P. R. China.
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