Document Detail


Athletes with exercise-associated fatigue have abnormally short muscle DNA telomeres.
MedLine Citation:
PMID:  12972872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION/PURPOSE: Although the beneficial health effects of regular moderate exercise are well established, there is substantial evidence that the heavy training and racing carried out by endurance athletes can cause skeletal muscle damage. This damage is repaired by satellite cells that can undergo a finite number of cell divisions. In this study, we have compared a marker of skeletal muscle regeneration of athletes with exercise-associated chronic fatigue, a condition labeled the "fatigued athlete myopathic syndrome" (FAMS), with healthy asymptomatic age- and mileage-matched control endurance athletes. METHODS: Muscle biopsies of the vastus lateralis were obtained from 13 patients diagnosed with FAMS and from 13 healthy control subjects. DNA was extracted from the muscle samples and their telomeric restriction fragment (TRF) or telomere lengths were measured by Southern blot analysis. RESULTS: All 13 symptomatic athletes reported a progressive decline in athletic performance, decreased ability to tolerate high mileage training, and excessive muscular fatigue during exercise. The minimum value of TRF lengths (4.0 +/- 1.8 kb) measured on the DNA from vastus lateralis biopsies from these athletes were significantly shorter than those from 13 age- and mileage-matched control athletes (5.4 +/- 0.6 kb, P < 0.05). Three of the FAMS patients had extremely short telomeres (1.0 +/- 0.3 kb). The minimum TRF lengths of the remaining 10 symptomatic athletes (4.9 +/- 0.5 kb, P < 0.05) were also significantly shorter that those of the control athletes. CONCLUSION: These findings suggest that skeletal muscle from symptomatic athletes with FAMS show extensive regeneration which most probably results from more frequent bouts of satellite cell proliferation in response to recurrent training- and racing-induced muscle injury.
Authors:
Malcolm Collins; Valerie Renault; Liesl A Grobler; Alan St Clair Gibson; Michael I Lambert; E Wayne Derman; Gillian S Butler-Browne; Timothy D Noakes; Vincent Mouly
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  35     ISSN:  0195-9131     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-15     Completed Date:  2004-01-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1524-8     Citation Subset:  IM; S    
Affiliation:
Department of Human Biology, University of Cape Town, South Africa. mcollins@sports.uct.ac.za
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MeSH Terms
Descriptor/Qualifier:
Adult
Biopsy
DNA
Exercise / physiology*
Fatigue / physiopathology*
Female
Humans
Male
Middle Aged
Muscle, Skeletal / injuries,  pathology
Physical Endurance
Sports
Syndrome
Telomere*
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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