| Atherosclerotic plaque composition: analysis with multicolor CT and targeted gold nanoparticles. | |
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MedLine Citation:
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PMID: 20668118 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To investigate the potential of spectral computed tomography (CT) (popularly referred to as multicolor CT), used in combination with a gold high-density lipoprotein nanoparticle contrast agent (Au-HDL), for characterization of macrophage burden, calcification, and stenosis of atherosclerotic plaques. MATERIALS AND METHODS: The local animal care committee approved all animal experiments. A preclinical spectral CT system in which incident x-rays are divided into six different energy bins was used for multicolor imaging. Au-HDL, an iodine-based contrast agent, and calcium phosphate were imaged in a variety of phantoms. Apolipoprotein E knockout (apo E-KO) mice were used as the model for atherosclerosis. Gold nanoparticles targeted to atherosclerosis (Au-HDL) were intravenously injected at a dose of 500 mg per kilogram of body weight. Iodine-based contrast material was injected 24 hours later, after which the mice were imaged. Wild-type mice were used as controls. Macrophage targeting by Au-HDL was further evaluated by using transmission electron microscopy and confocal microscopy of aorta sections. RESULTS: Multicolor CT enabled differentiation of Au-HDL, iodine-based contrast material, and calcium phosphate in the phantoms. Accumulations of Au-HDL were detected in the aortas of the apo E-KO mice, while the iodine-based contrast agent and the calcium-rich tissue could also be detected and thus facilitated visualization of the vasculature and bones (skeleton), respectively, during a single scanning examination. Microscopy revealed Au-HDL to be primarily localized in the macrophages on the aorta sections; hence, the multicolor CT images provided information about the macrophage burden. CONCLUSION: Spectral CT used with carefully chosen contrast agents may yield valuable information about atherosclerotic plaque composition. |
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Authors:
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David P Cormode; Ewald Roessl; Axel Thran; Torjus Skajaa; Ronald E Gordon; Jens-Peter Schlomka; Valentin Fuster; Edward A Fisher; Willem J M Mulder; Roland Proksa; Zahi A Fayad |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2010-07-28 |
Journal Detail:
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Title: Radiology Volume: 256 ISSN: 1527-1315 ISO Abbreviation: Radiology Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-19 Completed Date: 2010-09-30 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 0401260 Medline TA: Radiology Country: United States |
Other Details:
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Languages: eng Pagination: 774-82 Citation Subset: AIM; IM |
Copyright Information:
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(c) RSNA 2010. |
Affiliation:
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Translational and Molecular Imaging Institute, Mount Sinai School of Medicine, One Gustave L. Levy Place, New York, NY 10029, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aortic Diseases / pathology, radiography* Atherosclerosis / pathology, radiography* Color Contrast Media / diagnostic use Gold / diagnostic use* Iodine / diagnostic use Mice Mice, Knockout Microscopy, Confocal Microscopy, Electron Nanoparticles / diagnostic use* Phantoms, Imaging Tomography, X-Ray Computed / methods* |
| Grant Support | |
ID/Acronym/Agency:
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K99 EB012165-01/EB/NIBIB NIH HHS; R01 EB009638/EB/NIBIB NIH HHS; R01 EB009638-07/EB/NIBIB NIH HHS; R01 HL61814/HL/NHLBI NIH HHS; R01 HL71021/HL/NHLBI NIH HHS; R01 HL78667/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media; 7440-57-5/Gold; 7553-56-2/Iodine |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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