| AtBAG7, an Arabidopsis Bcl-2-associated athanogene, resides in the endoplasmic reticulum and is involved in the unfolded protein response. | |
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MedLine Citation:
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PMID: 20231441 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Bcl-2-associated athanogene (BAG) family is an evolutionarily conserved, multifunctional group of cochaperones that perform diverse cellular functions ranging from proliferation to growth arrest and cell death in yeast, in mammals, and, as recently observed, in plants. The Arabidopsis genome contains seven homologs of the BAG family, including four with domain organization similar to animal BAGs. In the present study we show that an Arabidopsis BAG, AtBAG7, is a uniquely localized endoplasmic reticulum (ER) BAG that is necessary for the proper maintenance of the unfolded protein response (UPR). AtBAG7 was shown to interact directly in vivo with the molecular chaperone, AtBiP2, by bimolecular fluorescence complementation assays, and the interaction was confirmed by yeast two-hybrid assay. Treatment with an inducer of UPR, tunicamycin, resulted in accelerated cell death of AtBAG7-null mutants. Furthermore, AtBAG7 knockouts were sensitive to known ER stress stimuli, heat and cold. In these knockouts heat sensitivity was reverted successfully to the wild-type phenotype with the addition of the chemical chaperone, tauroursodexycholic acid (TUDCA). Real-time PCR of ER stress proteins indicated that the expression of the heat-shock protein, AtBiP3, is selectively up-regulated in AtBAG7-null mutants upon heat and cold stress. Our results reveal an unexpected diversity of the plant's BAG gene family and suggest that AtBAG7 is an essential component of the UPR during heat and cold tolerance, thus confirming the cytoprotective role of plant BAGs. |
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Authors:
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Brett Williams; Mehdi Kabbage; Robert Britt; Martin B Dickman |
Publication Detail:
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Type: Journal Article Date: 2010-03-15 |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 ISSN: 1091-6490 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-04-01 Completed Date: 2010-04-28 Revised Date: 2010-09-30 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: United States |
Other Details:
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Languages: eng Pagination: 6088-93 Citation Subset: IM |
Affiliation:
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Department of Plant Pathology and Microbiology, Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX 77843, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics*,
metabolism* Arabidopsis Proteins / genetics*, metabolism* Cold Temperature Cryoprotective Agents / metabolism Endoplasmic Reticulum / metabolism* Genes, Plant* Genetic Complementation Test Hot Temperature Molecular Chaperones / genetics, metabolism Multigene Family Mutation Stress, Physiological Tunicamycin / pharmacology Two-Hybrid System Techniques Unfolded Protein Response / drug effects, genetics*, physiology* Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Cryoprotective Agents; 0/Molecular Chaperones; 11089-65-9/Tunicamycin |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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