Document Detail

AtAPY1 and AtAPY2 Function as Golgi localized Nucleoside Diphosphatases in Arabidopsis thaliana.
MedLine Citation:
PMID:  23034877     Owner:  NLM     Status:  Publisher    
NTPDases (Apyrases) (EC hydrolyze di- and triphosphate nucleotides, but not monophosphate nucleotides. They are categorized as E-type ATPases, have a broad divalent cation (Mg(2+), Ca(2+)) requirement for activation, and are insensitive to inhibitors of F-type, P-type, and V-type ATPases. Among the seven NTPDases identified in Arabidopsis, only APYRASE 1 (AtAPY1) and APYRASE 2 (AtAPY2) have been previously characterized. In this work, either AtAPY1 or AtAPY2 tagged with C-terminal green fluorescence protein (GFP) driven by their respective native promoter can rescue the apy1 apy2 double knockout (apy1 apy2 dKO) successfully, and confocal microscopy reveals that these two Arabidopsis apyrases reside in the Golgi apparatus. In Saccharomyces cerevisiae, both AtAPY1 and AtAPY2 can complement the Golgi-localized GDA1 mutant rescuing its aberrant protein glycosylation phenotype. In Arabidopsis, microsomes of wildtype show higher substrate preferences toward UDP compared to other NDP substrates. Loss-of-function Arabidopsis AtAPY1 mutants exhibit reduced microsomal UDPase activity, and this activity is even more significantly reduced in the loss-of-function AtAPY2 mutant and in the AtAPY1/AtAPY2 RNAi technology repressor lines. Microsomes from wildtype plants also have detectable GDPase activity, which is significantly reduced in apy2 but not apy1 mutants. The GFP tagged AtAPY1 or AtAPY2 constructs in the apy1 apy2 dKO plants can restore microsomal UDP/GDPase activity confirming that they both also have functional competency. The cell walls of apy1, apy2 and the RNAi silenced lines all have increased composition of galactose, but the transport efficiency of UDP-galactose across microsomal membranes was not altered. Taken together these results reveal that AtAPY1 and AtAPY2 are Golgi localized nucleotide diphosphatases and are likely to have roles in regulating UDP/GDP concentrations in the Golgi lumen.
Tsan-Yu Chiu; Katy Christiansen; Ignacio Moreno; Jeemeng Lao; Dominique Loqué; Ariel Orellana; Joshua L Heazlewood; Greg Clark; Stanley J Roux
Related Documents :
23763587 - Substrate and cell contact dependent inhibitor affinity of human organic cation transpo...
23248847 - High-level production of a cold-active b-mannanase from bacillus subtilis bs5 and its m...
24632627 - Identification of novel bacterial expansins and their synergistic actions on cellulose ...
24014027 - P87 and p101 subunits are distinct regulators determining class ib phosphoinositide 3-k...
20112037 - Quantum chemical studies on the inhibition potentials of some penicillin compounds for ...
3491067 - Fluorescence labeling of a single sulfhydryl group in human plasma alpha 1-proteinase i...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-3
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Section of Molecular Cell and Developmental Biology, University of Texas, Austin, Texas 78712, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A Strange Bullet which Caused Chylomediastinum and Chyloptysis.
Next Document:  Nonfunctionalization of two CYP82E nicotine N-demethylase genes abolishes nornicotine formation in N...