Document Detail

AtABCA9 transporter supplies fatty acids for lipid synthesis to the endoplasmic reticulum.
MedLine Citation:
PMID:  23269834     Owner:  NLM     Status:  MEDLINE    
Fatty acids, the building blocks of biological lipids, are synthesized in plastids and then transported to the endoplasmic reticulum (ER) for assimilation into specific lipid classes. The mechanism of fatty acid transport from plastids to the ER has not been identified. Here we report that AtABCA9, an ABC transporter in Arabidopsis thaliana, mediates this transport. AtABCA9 was localized to the ER, and atabca9 null mutations reduced seed triacylglycerol (TAG) content by 35% compared with WT. Developing atabca9 seeds incorporated 35% less (14)C-oleoyl-CoA into TAG compared with WT seeds. Furthermore, overexpression of AtABCA9 enhanced TAG deposition by up to 40%. These data strongly support a role for AtABCA9 as a supplier of fatty acid substrates for TAG biosynthesis at the ER during the seed-filling stage. AtABCA9 may be a powerful tool for increasing lipid production in oilseeds.
Sangwoo Kim; Yasuyo Yamaoka; Hirofumi Ono; Hanul Kim; Donghwan Shim; Masayoshi Maeshima; Enrico Martinoia; Edgar B Cahoon; Ikuo Nishida; Youngsook Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-12-26
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  110     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-09     Completed Date:  2013-03-07     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  773-8     Citation Subset:  IM    
Division of Molecular Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea.
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MeSH Terms
ATP-Binding Cassette Transporters / genetics,  metabolism*
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
DNA Primers / genetics
Endoplasmic Reticulum / metabolism*
Fatty Acids / metabolism*
Gene Expression Profiling
Gene Knockout Techniques
Lipids / biosynthesis*
Seeds / chemistry
Reg. No./Substance:
0/Arabidopsis Proteins; 0/DNA Primers; 0/Fatty Acids; 0/Lipids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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