| Asymmetry between sister cells in a cancer cell line revealed by chemical cytometry. | |
| | |
MedLine Citation:
|
PMID: 15228369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We introduce instrumentation and methodology for two-channel chemical cytometry of sister cells-two cells born from division of the same mother cell. The method is based on capillary electrophoresis with laser-induced fluorescence detection and allows simultaneously probing multiple intracellular components in sister cells. To test the new technology, we compared the expression patterns of green fluorescent protein (GFP) between the sisters in cultured cancer cells stably transfected with a GFP-expressing construct. We found that all sister cells had detectable asymmetry in the GFP expression patterns with a confidence level of higher than 95%. To our best knowledge, this is the first reported observation of asymmetric patterns of protein expression in sister cells in a cancer cell line. The proposed technology can reliably detect minor differences in chemical contents between sister cells, which makes it a potentially indispensable tool in studying the molecular mechanisms of developmental processes. It will be especially valuable in quantitative studies of cells with complex proliferation kinetics (e.g., stem cells). |
| | |
Authors:
|
Kang Hu; Hossein Ahmadzadeh; Sergey N Krylov |
Related Documents
:
|
7763479 - Selection of defined cell types by flow-cytometric cell sorting. 11226479 - Quantitative analysis of specific mrna species in minute cell samples by rt-pcr and flo... 20498909 - An efficient surface modification using 2-methacryloyloxyethyl phosphorylcholine to con... 19949979 - Development of a three-dimensional in vitro model for longitudinal observation of cell ... 18315499 - A one-day, dispense-only ip-one htrf assay for high-throughput screening of galphaq pro... 8879269 - Dna damage in exfoliated buccal cells of smokers assessed by the single cell gel electr... 15761969 - Changes of the gastric endocrine cells in the c57bl/6 mouse after implantation of murin... 8171319 - Genetic control of programmed cell death in drosophila. 1036399 - Nonhuman primate diploid cells for vaccine production. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Analytical chemistry Volume: 76 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2004 Jul |
Date Detail:
|
Created Date: 2004-07-01 Completed Date: 2005-05-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 3864-6 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Line, Tumor Cell Separation / methods* Electrophoresis, Capillary / methods Female Flow Cytometry / methods* Gene Expression Regulation, Neoplastic Green Fluorescent Proteins / analysis, biosynthesis, genetics Mammary Neoplasms, Experimental / genetics, metabolism*, pathology Sensitivity and Specificity Time Factors Transfection |
| Chemical | |
Reg. No./Substance:
|
147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plastic tip arrays for force spectroscopy.
Next Document: Anticipating the future: how the emergence of innovative biologic agents impacts benefit design, uti...