Document Detail


Asymmetry between sister cells in a cancer cell line revealed by chemical cytometry.
MedLine Citation:
PMID:  15228369     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We introduce instrumentation and methodology for two-channel chemical cytometry of sister cells-two cells born from division of the same mother cell. The method is based on capillary electrophoresis with laser-induced fluorescence detection and allows simultaneously probing multiple intracellular components in sister cells. To test the new technology, we compared the expression patterns of green fluorescent protein (GFP) between the sisters in cultured cancer cells stably transfected with a GFP-expressing construct. We found that all sister cells had detectable asymmetry in the GFP expression patterns with a confidence level of higher than 95%. To our best knowledge, this is the first reported observation of asymmetric patterns of protein expression in sister cells in a cancer cell line. The proposed technology can reliably detect minor differences in chemical contents between sister cells, which makes it a potentially indispensable tool in studying the molecular mechanisms of developmental processes. It will be especially valuable in quantitative studies of cells with complex proliferation kinetics (e.g., stem cells).
Authors:
Kang Hu; Hossein Ahmadzadeh; Sergey N Krylov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  76     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-07-01     Completed Date:  2005-05-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3864-6     Citation Subset:  IM    
Affiliation:
Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Cell Separation / methods*
Electrophoresis, Capillary / methods
Female
Flow Cytometry / methods*
Gene Expression Regulation, Neoplastic
Green Fluorescent Proteins / analysis,  biosynthesis,  genetics
Mammary Neoplasms, Experimental / genetics,  metabolism*,  pathology
Sensitivity and Specificity
Time Factors
Transfection
Chemical
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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