Document Detail


Asymmetrical localization and segregation of Paracentrotus lividus Bep4 maternal protein.
MedLine Citation:
PMID:  10495266     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asymmetric divisions that produce two distinct cells play a fundamental role in generating different cell types during development. Here we investigate the role of the cortex region and mitotic apparatus in asymmetrical localization and segregation of Bep4 protein in Paracentrotus lividus egg. By centrifugation of eggs with or without drugs we established an involvement of the cortex region in localization of Bep4 protein, confirmed by immunohistochemistry of isolated cortex. Association with the mitotic apparatus during cell division permits selective partitioning of Bep4 protein into the daughter cells. Direct association with spindle was also demonstrated both by Western blot and immunohistochemistry after isolation of the mitotic apparatus.
Authors:
D P Romancino; M Di Carlo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mechanisms of development     Volume:  87     ISSN:  0925-4773     ISO Abbreviation:  Mech. Dev.     Publication Date:  1999 Sep 
Date Detail:
Created Date:  2000-03-16     Completed Date:  2000-03-16     Revised Date:  2004-05-31    
Medline Journal Info:
Nlm Unique ID:  9101218     Medline TA:  Mech Dev     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  3-9     Citation Subset:  IM    
Affiliation:
Istituto di Biologia dello Sviluppo, CNR, via Ugo La Malfa, 153, Palermo, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Body Patterning
Cell Nucleus / metabolism
Cleavage Stage, Ovum / metabolism
Colchicine / pharmacology
Cytoskeleton / metabolism
Gene Expression Regulation, Developmental*
Immunohistochemistry
Membrane Proteins / genetics,  metabolism*
Microtubules / drug effects,  metabolism
Mitosis / drug effects
Ovum / metabolism
Sea Urchins
Chemical
Reg. No./Substance:
0/Bep4 protein, Paracentrotus lividus; 0/Membrane Proteins; 64-86-8/Colchicine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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