| Asymmetrical localization and segregation of Paracentrotus lividus Bep4 maternal protein. | |
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MedLine Citation:
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PMID: 10495266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Asymmetric divisions that produce two distinct cells play a fundamental role in generating different cell types during development. Here we investigate the role of the cortex region and mitotic apparatus in asymmetrical localization and segregation of Bep4 protein in Paracentrotus lividus egg. By centrifugation of eggs with or without drugs we established an involvement of the cortex region in localization of Bep4 protein, confirmed by immunohistochemistry of isolated cortex. Association with the mitotic apparatus during cell division permits selective partitioning of Bep4 protein into the daughter cells. Direct association with spindle was also demonstrated both by Western blot and immunohistochemistry after isolation of the mitotic apparatus. |
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Authors:
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D P Romancino; M Di Carlo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Mechanisms of development Volume: 87 ISSN: 0925-4773 ISO Abbreviation: Mech. Dev. Publication Date: 1999 Sep |
Date Detail:
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Created Date: 2000-03-16 Completed Date: 2000-03-16 Revised Date: 2004-05-31 |
Medline Journal Info:
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Nlm Unique ID: 9101218 Medline TA: Mech Dev Country: IRELAND |
Other Details:
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Languages: eng Pagination: 3-9 Citation Subset: IM |
Affiliation:
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Istituto di Biologia dello Sviluppo, CNR, via Ugo La Malfa, 153, Palermo, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Body Patterning Cell Nucleus / metabolism Cleavage Stage, Ovum / metabolism Colchicine / pharmacology Cytoskeleton / metabolism Gene Expression Regulation, Developmental* Immunohistochemistry Membrane Proteins / genetics, metabolism* Microtubules / drug effects, metabolism Mitosis / drug effects Ovum / metabolism Sea Urchins |
| Chemical | |
Reg. No./Substance:
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0/Bep4 protein, Paracentrotus lividus; 0/Membrane Proteins; 64-86-8/Colchicine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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