| Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division. | |
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MedLine Citation:
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PMID: 20522779 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) regulates cellular motility and the synthesis of organelles and molecules that promote adhesion to a variety of biological and nonbiological surfaces. These properties likely require tight spatial and temporal regulation of c-di-GMP concentration. We have developed genetically encoded fluorescence resonance energy transfer (FRET)-based biosensors to monitor c-di-GMP concentrations within single bacterial cells by microscopy. Fluctuations of c-di-GMP were visualized in diverse Gram-negative bacterial species and observed to be cell cycle dependent. Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa. Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function. |
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Authors:
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Matthias Christen; Hemantha D Kulasekara; Beat Christen; Bridget R Kulasekara; Lucas R Hoffman; Samuel I Miller |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Science (New York, N.Y.) Volume: 328 ISSN: 1095-9203 ISO Abbreviation: Science Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-06-04 Completed Date: 2010-06-16 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404511 Medline TA: Science Country: United States |
Other Details:
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Languages: eng Pagination: 1295-7 Citation Subset: IM |
Affiliation:
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Department of Immunology, University of Washington, Seattle, WA 98195, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biosensing Techniques Caulobacter crescentus / cytology*, genetics, metabolism* Cell Division* Cyclic GMP / analogs & derivatives*, metabolism Fluorescence Resonance Energy Transfer Klebsiella pneumoniae / cytology, metabolism Microscopy Movement Mutation Phosphorus-Oxygen Lyases / genetics, metabolism Pseudomonas aeruginosa / cytology*, genetics, metabolism* Salmonella typhimurium / cytology, metabolism Second Messenger Systems* |
| Grant Support | |
ID/Acronym/Agency:
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1R21NS067579-01/NS/NINDS NIH HHS; K08AI066251/AI/NIAID NIH HHS; U54AI057141/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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61093-23-0/bis(3',5')-cyclic diguanylic acid; 7665-99-8/Cyclic GMP; EC 4.6.-/Phosphorus-Oxygen Lyases; EC 4.6.1.-/diguanylate cyclase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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