Document Detail


Asymmetrical distribution of the second messenger c-di-GMP upon bacterial cell division.
MedLine Citation:
PMID:  20522779     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The bacterial second messenger cyclic diguanosine monophosphate (c-di-GMP) regulates cellular motility and the synthesis of organelles and molecules that promote adhesion to a variety of biological and nonbiological surfaces. These properties likely require tight spatial and temporal regulation of c-di-GMP concentration. We have developed genetically encoded fluorescence resonance energy transfer (FRET)-based biosensors to monitor c-di-GMP concentrations within single bacterial cells by microscopy. Fluctuations of c-di-GMP were visualized in diverse Gram-negative bacterial species and observed to be cell cycle dependent. Asymmetrical distribution of c-di-GMP in the progeny correlated with the time of cell division and polarization for Caulobacter crescentus and Pseudomonas aeruginosa. Thus, asymmetrical distribution of c-di-GMP was observed as part of cell division, which may indicate an important regulatory step in extracellular organelle biosynthesis or function.
Authors:
Matthias Christen; Hemantha D Kulasekara; Beat Christen; Bridget R Kulasekara; Lucas R Hoffman; Samuel I Miller
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  328     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-04     Completed Date:  2010-06-16     Revised Date:  2014-02-28    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1295-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques
Caulobacter crescentus / cytology*,  genetics,  metabolism*
Cell Division*
Cyclic GMP / analogs & derivatives*,  metabolism
Escherichia coli Proteins
Fluorescence Resonance Energy Transfer
Klebsiella pneumoniae / cytology,  metabolism
Microscopy
Movement
Mutation
Phosphorus-Oxygen Lyases / genetics,  metabolism
Pseudomonas aeruginosa / cytology*,  genetics,  metabolism*
Salmonella typhimurium / cytology,  metabolism
Second Messenger Systems*
Grant Support
ID/Acronym/Agency:
1R21NS067579-01/NS/NINDS NIH HHS; K02 HL105543/HL/NHLBI NIH HHS; K08 AI066251/AI/NIAID NIH HHS; K08AI066251/AI/NIAID NIH HHS; R01 HL098084/HL/NHLBI NIH HHS; U54AI057141/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Escherichia coli Proteins; 61093-23-0/bis(3',5')-cyclic diguanylic acid; EC 4.6.-/Phosphorus-Oxygen Lyases; EC 4.6.1.-/diguanylate cyclase; H2D2X058MU/Cyclic GMP
Comments/Corrections

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