Document Detail


Asymmetric transcription of R plasmid NR1 in Proteus mirabilis.
MedLine Citation:
PMID:  378944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The composite R plasmid NR1, its resistance transfer factor which specifies resistance to tetracycline (RTF-Tc component), and its r-determinants component were each denatured and centrifuged to equilibrium in CsCl density gradients containing polyuridylic acid-polyguanidylic acid. The complementary deoxyribonucleic acid strands of NR1 and the complementary strands of the RTF-Tc component could be separated by this technique because of a threefold difference in polyuridylic acid-polyguanidylic acid binding to the strands of the RTF-Tc component. The two strands of the r-determinants component bound equal amounts of polyuridylic acid-polyguanidylic acid. Hybridization of single strands of plasmid deoxyribonucleic acid with in vivo-labeled ribonucleic acid from Proteus mirabilis containing NR1 indicated that transcription within the RTF-Tc component is from the NR1 strand which preferentially binds polyuridylic acid-polyguanidylic acid, whereas transcription within the r-determinants component is predominantly from the complementary strand.
Authors:
E R Appelbaum; R H Rownd
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  138     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-09-27     Completed Date:  1979-09-27     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  878-83     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
DNA, Bacterial / genetics
DNA, Circular / genetics
Nucleic Acid Hybridization
Proteus mirabilis / drug effects,  genetics*
R Factors*
RNA, Bacterial / biosynthesis
Tetracycline / pharmacology
Transcription, Genetic*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/DNA, Circular; 0/RNA, Bacterial; 60-54-8/Tetracycline
Comments/Corrections

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