Document Detail

Asymmetric elastic properties of Dictyostelium discoideum in relation to chemotaxis.
MedLine Citation:
PMID:  17661497     Owner:  NLM     Status:  MEDLINE    
In this study we used an AFM to investigate the cytoskeletal properties of live Dictyostelium discoideum cells by measuring the local stiffness across individual living cells. We have examined differences in elastic properties of polarized and unpolarized AX3 wild type and the mutant DAip1- cells, as well as the differences in the front and rear of the cells in relation to organization of the actin cytoskeleton. We found that the average Young's modulus increases upon polarization for the thin regions of the cell and that in polarized cells, the cell front was stiffer than the cell back. We also found that AX3 cells were stiffer than DAip1- cells. This finding suggests that actin polymerization is one of the major determinants of cell motility in Dictyostelium. In addition, a thin agarose film was studied as a model system to examine the influence of the substrate of thin materials probed with the AFM.
Belinda J Haupt; Matthew Osbourn; Rudolph Spanhoff; Sandra de Keijzer; Annette Müller-Taubenberger; Ewa Snaar-Jagalska; Thomas Schmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-07-28
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  23     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2007 Aug 
Date Detail:
Created Date:  2007-08-21     Completed Date:  2007-10-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9352-7     Citation Subset:  IM    
Physics of Life Processes, Leiden Institute of Physics, Leiden University, The Netherlands.
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MeSH Terms
Cell Polarity
Dictyostelium / cytology*,  ultrastructure*
Microscopy, Atomic Force
Sepharose / chemistry
Reg. No./Substance:

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