Document Detail


Asymmetric cortical extension shifts cleavage furrow position in Drosophila neuroblasts.
MedLine Citation:
PMID:  21937716     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The cytokinetic cleavage furrow is typically positioned symmetrically relative to the cortical cell boundaries but can also be asymmetric. The mechanisms that control furrow site specification have been intensively studied but how polar cortex movements influence ultimate furrow position remains poorly understood. We measured the position of the apical and the basal cortex in asymmetrically dividing Drosophila neuroblasts and observed preferential displacement of the apical cortex that becomes the larger daughter cell during anaphase, effectively shifting the cleavage furrow towards the smaller daughter cell. Asymmetric cortical extension is correlated with the presence of cortical myosin II, which is polarized in neuroblasts. Loss of myosin II asymmetry by perturbing heterotrimeric G-protein signaling results in symmetric extension and equal sized daughter cells. We propose a model in which contraction-driven asymmetric polar extension of the neuroblast cortex during anaphase contributes to asymmetric furrow position and daughter cell size.
Authors:
Marisa Connell; Clemens Cabernard; Derek Ricketson; Chris Q Doe; Kenneth E Prehoda
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-9-21
Journal Detail:
Title:  Molecular biology of the cell     Volume:  -     ISSN:  1939-4586     ISO Abbreviation:  -     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-9-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Institute of Molecular Biology and Department of Chemistry, University of Oregon, Eugene, OR 97403, USA Institute of Molecular Biology and Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA.
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