Document Detail

Asymmetric chitosan membrane containing collagen I nanospheres for skin tissue engineering.
MedLine Citation:
PMID:  19419166     Owner:  NLM     Status:  MEDLINE    
A biodegradable chitosan membrane with an asymmetric structure, seeded with fibroblasts, was prepared as a novel skin substitute. Chitosan was cross-linked with genipin and then frozen and lyophilized to yield a porous asymmetric membrane (CG membrane). Nanoscale collagen I particles were injected into the CG membrane to form an asymmetric CGC membrane. The results reveal that the CG membrane treated with 0.125 wt % of genipin had a higher swelling ratio, porosity, and pore size. After 7 d of dynamic culture, many of the adhered cells exhibited a flat morphology and well spread on the surface of CGC membrane treated with 0.125 wt % of genipin. In animal studies, the CGC membrane seeded with fibroblasts and grown in vitro for 7 d was more effective than both gauze and commercial wound dressing, Suile, in healing wounds. An in vivo histological assessment indicated that covering the wound with the asymmetric CGC membrane resulted in its epithelialization and reconstruction. CGC membrane, thus, has great potential in skin tissue engineering.
Kuo-Yu Chen; Wei-Ju Liao; Shyh-Ming Kuo; Fuu-Jen Tsai; Yueh-Sheng Chen; Chih-Yang Huang; Chun-Hsu Yao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomacromolecules     Volume:  10     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-08     Completed Date:  2009-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1642-9     Citation Subset:  IM    
Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
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MeSH Terms
Cells, Cultured
Chitosan / chemistry*
Collagen Type I / chemistry*
Microscopy, Electron, Scanning
Rats, Sprague-Dawley
Skin / cytology
Skin, Artificial*
Tissue Engineering*
Reg. No./Substance:
0/Collagen Type I; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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