Document Detail


Asymmetric segregation of the double-stranded RNA binding protein Staufen2 during mammalian neural stem cell divisions promotes lineage progression.
MedLine Citation:
PMID:  22902295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asymmetric cell divisions are a fundamental feature of neural development, and misregulation can lead to brain abnormalities or tumor formation. During an asymmetric cell division, molecular determinants are segregated preferentially into one daughter cell to specify its fate. An important goal is to identify the asymmetric determinants in neural progenitor cells, which could be tumor suppressors or inducers of specific neural fates. Here, we show that the double-stranded RNA-binding protein Stau2 is distributed asymmetrically during progenitor divisions in the developing mouse cortex, preferentially segregating into the Tbr2(+) neuroblast daughter, taking with it a subset of RNAs. Knockdown of Stau2 stimulates differentiation and overexpression produces periventricular neuronal masses, demonstrating its functional importance for normal cortical development. We immunoprecipitated Stau2 to examine its cargo mRNAs, and found enrichment for known asymmetric and basal cell determinants, such as Trim32, and identified candidates, including a subset involved in primary cilium function.
Authors:
Gretchen Kusek; Melissa Campbell; Frank Doyle; Scott A Tenenbaum; Michael Kiebler; Sally Temple
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  Cell stem cell     Volume:  11     ISSN:  1875-9777     ISO Abbreviation:  Cell Stem Cell     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-08     Completed Date:  2013-03-07     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  101311472     Medline TA:  Cell Stem Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  505-16     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Inc. All rights reserved.
Affiliation:
Neural Stem Cell Institute, Regenerative Research Foundation, Rensselaer, NY 12144, USA.
Data Bank Information
Bank Name/Acc. No.:
GEO/GSE38222
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MeSH Terms
Descriptor/Qualifier:
Animals
Asymmetric Cell Division*
Cell Differentiation / genetics
Cell Lineage / genetics
Cells, Cultured
Cerebellar Cortex / embryology*,  metabolism
Female
Gene Expression Regulation, Developmental
Mice
Mice, Knockout
Microarray Analysis
Nerve Tissue Proteins / genetics,  metabolism*
RNA, Double-Stranded / metabolism
RNA-Binding Proteins / genetics,  metabolism*
Ubiquitin-Protein Ligases / genetics,  metabolism
Grant Support
ID/Acronym/Agency:
F32 NS061461/NS/NINDS NIH HHS; F32 NS061461/NS/NINDS NIH HHS; R01 NS074047/NS/NINDS NIH HHS; R01NS074047/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Nerve Tissue Proteins; 0/RNA, Double-Stranded; 0/RNA-Binding Proteins; 0/stau2 protein, mouse; EC 6.3.2.19/TRIM32 protein, mouse; EC 6.3.2.19/Ubiquitin-Protein Ligases
Comments/Corrections

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