Document Detail


Astrocytic amino acid metabolism under control conditions and during oxygen and/or glucose deprivation.
MedLine Citation:
PMID:  12608698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Amino acid contents were measured in 1- and 3-week-old primary cultures of astrocytes and in their incubation media, an amino acid-free salt solution with or without glucose, during 3-h incubation under normoxic or anoxic conditions. Most essential amino acids were rapidly released to the medium during the beginning of the incubation. A subsequent slow medium increase reflected proteolysis. Glutamate and aspartate were absent from the media during all conditions, indicating fueling of their uptake by either glycolytically or oxidatively derived energy. The total content of glutamine increased, except during incubation in glucose-deprived media, when it declined or remained constant. Changes in aspartate were negligible, suggesting oxidative degradation of aspartate-derived oxaloacetate during normoxia and its reduction to succinate during anoxia, driving regeneration of NAD+ from NADH. An increase of alanine was reduced in glucose-free media, whereas serine showed especially large increase during isolated glucose deprivation, suggesting its production from glutamine via 3-phosphoglycerate.
Authors:
Leif Hertz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  28     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-02-28     Completed Date:  2003-04-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  243-58     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada. lhertz@northcom.net
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Astrocytes / metabolism*
Cells, Cultured
Culture Media
Glucose / deficiency*
Mice
Oxygen / metabolism*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 50-99-7/Glucose; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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