| Astrocytes contacting HIV-1-infected macrophages increase the release of CCL2 in response to the HIV-1-dependent enhancement of membrane-associated TNFα in macrophages. | |
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MedLine Citation:
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PMID: 20737475 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of human immunodeficiency virus (HIV)-infected macrophages in the parenchyma of central nervous system is an hallmark of acquired immunodeficiency syndrome-related neuroinflammation. Once penetrated the blood-brain barrier (BBB), macrophages closely interact with astrocytes, beginning with those lying beneath the BBB endothelium. By investigating the consequences of the cell-cell interaction between HIV-infected macrophages and astrocytes, we observed that the HIV-1 expression in macrophagic cells correlated with increased chemotactic activity in supernatants of astroglial cells. Gene array analysis revealed an impressive increase in the transcription of the gene for the CCL2/MCP-1 chemokine in astroglial cells isolated from HIV-1-infected co-cultures compared with cells from uninfected co-cultures. This phenomenon coupled with the increase in CCL2 release and depended on the cell-cell contact. In addition, it was a consequence of the HIV-1-induced enhancement of membrane-associated tumor necrosis factor-α in macrophagic cells, and correlated with increased levels of nuclear factor kappaB activation in astroglial cells. These observations could mirror a mechanism of recruitment of leukocytes through the BBB, likely contributing to the increase in both viral load and inflammation in central nervous system of HIV-infected patients. |
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Authors:
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Claudia Muratori; Giorgio Mangino; Elisabetta Affabris; Maurizio Federico |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glia Volume: 58 ISSN: 1098-1136 ISO Abbreviation: Glia Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-10-20 Completed Date: 2011-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8806785 Medline TA: Glia Country: United States |
Other Details:
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Languages: eng Pagination: 1893-904 Citation Subset: IM |
Affiliation:
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National AIDS Center, Istituto Superiore di Sanità, Rome, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Astrocytes
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immunology,
pathology,
virology* Astrocytoma Brain Neoplasms Cell Communication / immunology Chemokine CCL2 / genetics, immunology* Chemotaxis / immunology Coculture Techniques Gene Expression / immunology HIV Infections / immunology*, pathology HIV-1* Humans I-kappa B Proteins / metabolism Macrophages / immunology, pathology, virology* Membrane Proteins / immunology, metabolism Oligonucleotide Array Sequence Analysis Tumor Necrosis Factor-alpha / immunology*, metabolism U937 Cells |
| Chemical | |
Reg. No./Substance:
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0/CCL2 protein, human; 0/Chemokine CCL2; 0/I-kappa B Proteins; 0/Membrane Proteins; 0/Tumor Necrosis Factor-alpha; 139874-52-5/NF-kappaB inhibitor alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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