Document Detail


Astrocyte and macrophage regulation of YKL-40 expression and cellular response in neuroinflammation.
MedLine Citation:
PMID:  22074331     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous inflammatory conditions are associated with elevated YKL-40 expression by infiltrating macrophages. Thus, we were surprised to observe minimal macrophage and abundant astrocyte expression of YKL-40 in neuroinflammatory conditions. The aims of the current study were to better delineate this discrepancy, characterize the factors that regulate YKL-40 expression in macrophages and astrocytes and study whether YKL-40 expression correlates with cell morphology and/or activation state. In vitro, macrophages expressed high levels of YKL-40 that was induced by classical activation and inhibited by alternative activation. Cytokines released from macrophages induced YKL-40 transcription in astrocytes that was accompanied by morphological changes and altered astrocytic motility. Because coculturing of astrocytes and macrophages did not reverse this in vitro expression pattern, additional components of the in vivo central nervous system (CNS) milieu must be required to suppress macrophage and induce astrocyte expression of YKL-40.
Authors:
Dafna Bonneh-Barkay; Stephanie J Bissel; Julia Kofler; Adam Starkey; Guoji Wang; Clayton A Wiley
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-12-22
Journal Detail:
Title:  Brain pathology (Zurich, Switzerland)     Volume:  22     ISSN:  1750-3639     ISO Abbreviation:  Brain Pathol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-15     Completed Date:  2012-10-12     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9216781     Medline TA:  Brain Pathol     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  530-46     Citation Subset:  IM    
Copyright Information:
© 2011 The Authors; Brain Pathology © 2011 International Society of Neuropathology.
Affiliation:
Department of Pathology, University of Pittsburgh, PA, USA. dafnabb@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Adipokines / biosynthesis*
Astrocytes / metabolism*
Blotting, Western
Brain / metabolism*,  pathology
Coculture Techniques
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fluorescent Antibody Technique
Humans
In Situ Hybridization
Inflammation / metabolism,  pathology
Lectins / biosynthesis*
Macrophages / metabolism*
Real-Time Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
K24 MH01717/MH/NIMH NIH HHS; R01 MH071151/MH/NIMH NIH HHS; R01 MH071151/MH/NIMH NIH HHS; R21 AG025829/AG/NIA NIH HHS
Chemical
Reg. No./Substance:
0/Adipokines; 0/CHI3L1 protein, human; 0/Lectins
Comments/Corrections

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