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Associations of LINE-1 DNA Methylation with Preterm Birth in a Prospective Cohort Study.
MedLine Citation:
PMID:  22720130     Owner:  NLM     Status:  Publisher    
Preterm birth affects over 12% of all infants born in the US yet the biology of early delivery remains unclear, including whether epigenetic mechanisms are involved. We examined associations of maternal and umbilical cord blood long interspersed nuclear element-1 (LINE-1) DNA methylation with length of gestation and odds of preterm birth in singleton pregnancies in Project Viva. In white blood cells from maternal blood during 1(st) trimester (n=914) and 2(nd) trimester (n=922), and from venous cord blood at delivery (n=557), we measured LINE-1 by pyrosequencing (expressed as %5 methyl cytosines within the LINE-1 region analyzed [%5mC]). We ran linear regression models to analyze differences in gestation length, and logistic models for odds of preterm birth (<37 v. ≥37 weeks gestation), across quartiles of LINE-1. Mean(SD) LINE-1 levels were 84.3(0.6), 84.5(0.4), and 84.6(0.7) %5mC for 1(st) trimester, 2(nd) trimester and cord blood, respectively. Mean(SD) gestational age was 39.5(1.8) weeks, and 6.5% of infants were born preterm. After adjustment for maternal age, race/ethnicity, BMI, education, smoking status, and fetal sex, women with the highest vs. lowest quartile of 1(st) trimester LINE-1 had longer gestations (0.45 weeks [95% CI 0.12, 0.78]) and lower odds of preterm birth (OR 0.40 [0.17, 0.94]), whereas associations with cord blood LINE-1 were in the opposite direction (-0.45 weeks, -0.83, -0.08) and (OR 4.55 [1.18, 17.5]). In conclusion, higher early pregnancy LINE-1 predicts lower risk of preterm birth. In contrast, preterm birth is associated with lower LINE-1 in cord blood.
Heather H Burris; Sheryl L Rifas-Shiman; Andrea Baccarelli; Letizia Tarantini; Caroline E Boeke; Ken Kleinman; Augusto A Litonjua; Janet W Rich-Edwards; Matthew W Gillman
Publication Detail:
Journal Detail:
Title:  Journal of developmental origins of health and disease     Volume:  3     ISSN:  2040-1752     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101517692     Medline TA:  J Dev Orig Health Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  173-181     Citation Subset:  -    
Department of Neonatology, Beth Israel Deaconess Medical Center, Division of Newborn Medicine, Children's Hospital Boston, Harvard Medical School, Boston, MA, USA.
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