Document Detail


Association of tumor necrosis factor receptor type II polymorphism 196R with Systemic lupus erythematosus in the Japanese: molecular and functional analysis.
MedLine Citation:
PMID:  11762942     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate whether a polymorphism(s) or mutation(s) in the tumor necrosis factor receptor II (TNFRII) gene is involved in the pathogenesis of systemic lupus erythematosus (SLE). METHODS: All 10 exons of the TNFRII gene were analyzed by exon-specific polymerase chain reaction-single-strand conformation polymorphism, followed by nucleotide sequencing of exons that displayed aberrant bands. To analyze the function of the TNFRII polymorphisms, the full-length TNFRII complementary DNA of each allele was transfected in HeLa cells and then studied for specific binding of 125I-TNFalpha, as well as interleukin-6 (IL-6) production and cytotoxic activity after treatment with recombinant human TNFalpha. RESULTS: We identified 4 polymorphisms, at codons 56, 181, 196, and 232. The latter 2 had amino acid substitutions M196R and E232K, respectively. Only the 196R allele was significantly associated with SLE in our 105 Japanese SLE patients, with an allele frequency of 20.5%, compared with 12.6% in 99 healthy controls (P = 0.0335). More importantly, using TNFRII-transfected HeLa cells, we demonstrated significantly increased IL-6 production by 196R TNFRII compared with 196M TNFRII. The cytotoxic activity induced by 196R TNFRII was also increased compared with that of 196M TNFRII. This increase was achieved without affecting the binding affinity of TNFalpha to TNF-RII, as demonstrated by the finding that specific TNFalpha binding to the HeLa transfectants of 196R and 196M TNFRII was similar, with Kd values of 3.12 x 10(-10)M and 4.34 x 10(-10)M, respectively. CONCLUSION: These results suggest that 196R TNFRII, which transduces the signals of TNFalpha more effectively than does 196M TNFRII, is involved in the pathogenesis of SLE.
Authors:
C Morita; T Horiuchi; H Tsukamoto; N Hatta; Y Kikuchi; Y Arinobu; T Otsuka; T Sawabe; S Harashima; K Nagasawa; Y Niho
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Arthritis and rheumatism     Volume:  44     ISSN:  0004-3591     ISO Abbreviation:  Arthritis Rheum.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-13     Completed Date:  2002-01-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370605     Medline TA:  Arthritis Rheum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2819-27     Citation Subset:  AIM; IM    
Affiliation:
Kyushu University Graduate School of Medical Sciences, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Amino Acid Substitution / genetics
Antigens, CD / analysis,  genetics*,  metabolism*
Culture Media / chemistry
Female
Gene Expression
Gene Frequency
Genotype
Hela Cells
Humans
Interleukin-6 / biosynthesis
Iodine Radioisotopes / diagnostic use
Japan
Leukocytes, Mononuclear / physiology
Lupus Erythematosus, Systemic / genetics*
Male
Middle Aged
Phenotype
Polymorphism, Single-Stranded Conformational*
Protein Binding / genetics
Receptors, Tumor Necrosis Factor / analysis,  genetics*,  metabolism*
Receptors, Tumor Necrosis Factor, Type II
Solubility
Transfection
Tumor Necrosis Factor-alpha / metabolism,  pharmacology
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Culture Media; 0/Interleukin-6; 0/Iodine Radioisotopes; 0/Receptors, Tumor Necrosis Factor; 0/Receptors, Tumor Necrosis Factor, Type II; 0/Tumor Necrosis Factor-alpha

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