Document Detail


Association states of androgen receptors in nuclei of human benign hypertrophic prostate.
MedLine Citation:
PMID:  2423996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Androgen receptors (AR) were quantified in nuclei purified from unfractionated benign hypertrophic prostate (bph) tissue and from separated epithelium and stroma from bph specimens. Both epithelial and stromal cell nuclei contained AR, although concentrations in epithelial cell nuclei were higher and more variable. Variations in AR levels in epithelial cell nuclei reflected variations in unfractionated-tissue nuclei. Nuclear AR were further characterized regarding extractability with or resistance to 0.6 mol/lKCl and micrococcal nuclease. Nuclei from unfractionated tissue, epithelium, and stroma contained populations of AR susceptible and refractory to solubilization with KC1 and nuclease. Nuclease- and salt-sensitive populations of AR were similar numerically. The observed variability in epithelial cell nuclear AR was attributable to a wide range of solubilizable AR. Nuclease-digestion profiles and sedimentation analyses revealed that this wide range was not due to AR associated with soluble chromatin oligomers but to AR not detectably associated with other nuclear components. In contrast, AR in stromal cell nuclei was predominantly resistant to KC1 and nuclease, and variability in total nuclear AR concentration was due to variation in the nonextractable population.
Authors:
N Kyprianou; P Davies
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Prostate     Volume:  8     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  1986  
Date Detail:
Created Date:  1986-07-10     Completed Date:  1986-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  363-80     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Fractionation
Cell Nucleus / metabolism*
DNA / analysis
Dihydrotestosterone / metabolism
Humans
Male
Prostate / analysis
Prostatic Hyperplasia / metabolism*
Receptors, Androgen / analysis,  metabolism*
Chemical
Reg. No./Substance:
0/Receptors, Androgen; 521-18-6/Dihydrotestosterone; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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