| Association of the prion protein and its expression with ovarian follicle development in cattle. | |
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MedLine Citation:
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PMID: 17595008 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cellular form of the prion protein (PrP(C)) has been detected in many tissues including reproductive tissues. While its function is unclear, it has been suggested to act as a receptor for an unidentified ligand and/or as an antioxidant agent. We tested the hypothesis that PrP(C) is differentially expressed in dominant, growing, compared to subordinate bovine ovarian follicles. Using both microarray analysis and quantitative real-time PCR, the level of prion protein mRNA (Prnp) in both theca and granulosa cells was measured. We found that levels of Prnp were significantly higher in the theca cells of dominant compared to subordinate follicles but similar among granulosa cells from different follicles. This difference was apparent immediately after selection of the dominant follicle and continued to the dominance stage of the follicle wave. Levels of the protein for PrP(C) were also higher (P < 0.05) in theca cells of dominant compared to subordinate follicles. In conclusion, elevated PrP(C) was associated with ovarian follicle growth and development and we suggest that it may play a role in the success of follicle development. |
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Authors:
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N Forde; M Rogers; M J Canty; P Lonergan; G W Smith; P M Coussens; J J Ireland; A C O Evans |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular reproduction and development Volume: 75 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2007-12-06 Completed Date: 2008-05-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: United States |
Other Details:
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Languages: eng Pagination: 243-9 Citation Subset: IM |
Copyright Information:
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2007 Wiley-Liss, Inc |
Affiliation:
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School of Agriculture Food Science and Veterinary Medicine and the Conway Institute for Biomolecular and Biomedical Research, College of Life Sciences, University College Dublin, Belfield, Dublin, Ireland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Female Follicular Fluid / physiology Gene Expression Regulation* Oligonucleotide Array Sequence Analysis Ovarian Follicle / physiology* Ovary / physiology* PrPC Proteins / genetics* Prions / genetics* Reverse Transcriptase Polymerase Chain Reaction Swine |
| Chemical | |
Reg. No./Substance:
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0/PrPC Proteins; 0/Prions |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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