Document Detail


Association of the peroxisome proliferator-activated receptor α gene L162V polymorphism with stage C heart failure.
MedLine Citation:
PMID:  21430558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To analyze whether genetic variants of PPARA are associated with the development of stage C heart failure.
METHODS: We analyzed the distribution of the rs1800206, rs4253778 and rs135551 polymorphisms in genomic DNA extracted from peripheral blood cells of 534 patients in different heart failure stages and 63 healthy individuals. The mRNA expression of the peroxisome proliferator-activated receptor (PPAR)α target genes long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) and medium-chain acyl-CoA dehydrogenase (MCAD) was measured in myocardial biopsies of a subgroup of stage B and C patients. Functional studies were performed in HL-1 cardiomyocytes.
RESULTS: The V162 allele of the rs1800206 polymorphism was more frequent in stage C patients than in stage A and B patients and healthy individuals. Patients with the V162 allele exhibited decreased myocardial LCHAD and MCAD mRNA expression as compared to L162 homozygote patients. In addition, stage C patients exhibited lower myocardial LCHAD and MCAD mRNA expression than stage B patients. Cardiomyocytes transfected with the V162 allele presented decreased PPARα transcriptional activity, LCHAD mRNA expression and ATP production compared to cardiomyocytes transfected with the L162 variant.
CONCLUSIONS: These findings suggest that the V162 allele of the human PPARA gene can be a new risk factor in the development of stage C heart failure, likely via depressed cardiac PPARα activity.
Authors:
Teresa Arias; Javier Beaumont; Begoña López; Guillermo Zalba; Oscar Beloqui; Joaquín Barba; Félix Valencia; Juan José Gómez-Doblas; Eduardo De Teresa; Javier Díez
Related Documents :
12396248 - The impact of phorbol ester on the regulation of amiloride-sensitive epithelial sodium ...
15181158 - Expression of several cytoskeletal proteins in ovine cerebral arteries: developmental a...
9705268 - Altered expression and assembly of n-type calcium channel alpha1b and beta subunits in ...
17709608 - Synaptotagmin vii splice variants alpha, beta, and delta are expressed in pancreatic be...
17588558 - Bhlh transcription factors regulate organ morphogenesis via activation of an adamts pro...
15219628 - Transfer of maternally injected endocrine disruptors through breast milk during lactati...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of hypertension     Volume:  29     ISSN:  1473-5598     ISO Abbreviation:  J. Hypertens.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-04-08     Completed Date:  2011-07-29     Revised Date:  2011-08-31    
Medline Journal Info:
Nlm Unique ID:  8306882     Medline TA:  J Hypertens     Country:  England    
Other Details:
Languages:  eng     Pagination:  876-83     Citation Subset:  IM    
Copyright Information:
© 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins
Affiliation:
Division of Cardiovascular Sciences, Centre for Applied Medical Research, University of Navarra, Pamplona, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acyl-CoA Dehydrogenase / genetics
Base Sequence
DNA Primers
Echocardiography
Female
Heart Failure / genetics*,  ultrasonography
Humans
Male
Middle Aged
PPAR alpha / genetics*
Plasmids
Polymorphism, Genetic*
RNA, Messenger / genetics
Chemical
Reg. No./Substance:
0/DNA Primers; 0/PPAR alpha; 0/RNA, Messenger; EC 1.3.99.3/Acyl-CoA Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Heterogeneity in antihypertensive treatment discontinuation between drugs belonging to the same clas...
Next Document:  Associations of maternal obesity with blood pressure and the risks of gestational hypertensive disor...