Document Detail


Association of kinesin and myosin with pigment granules in crustacean chromatophores.
MedLine Citation:
PMID:  16420248     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chromatic adaptation in crustaceans results from the differential distribution of colored pigment granules within their chromatophores consequent to cell signaling by neurosecretory peptides. However, the force transducing, mechanochemical protein motors responsible for granule translocation, and their molecular mechanisms of action, are not well understood. The present study uses immunocytochemical techniques and a motility assay in vitro to demonstrate that protein motors from the kinesin and myosin superfamilies are stably associated with membrane-bounded pigment granules in the red, ovarian chromatophores of the freshwater, palaemonid shrimp, Macrobrachium olfersii. Monoclonal antibodies against conventional kinesin heavy chain, and an anti-myosin whole serum, labeled pigment-containing fragments prepared from homogenates of chromatophores with fully dispersed or aggregated pigments: this finding infers a permanent association between the protein motors and the pigment granules, and suggests that such motors may be regulated while bound to their cargos. The pigment aggregator appears to be a myosin since the anti-myosin whole serum attenuated hormonally triggered pigment aggregation in the motility assay in vitro, and induced pigment hyper-dispersion in some chromatophores. Western blots of the chromatophore-containing, ovarian tissue homogenate demonstrated protein bands consistent with myosin II and myosin XII, either of which may be the pigment aggregator. This study provides the first direct evidence for myosin and kinesin protein motors directly and stably associated with pigment granules in crustacean chromatophores, and may represent the first successful isolation of myosin class XII.
Authors:
Robert Tew Boyle; John Campbell McNamara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society     Volume:  19     ISSN:  0893-5785     ISO Abbreviation:  Pigment Cell Res.     Publication Date:  2006 Feb 
Date Detail:
Created Date:  2006-01-19     Completed Date:  2006-04-14     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8800247     Medline TA:  Pigment Cell Res     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  68-75     Citation Subset:  IM    
Affiliation:
Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Chromatophores / chemistry,  metabolism*
Cytoplasmic Granules / metabolism*
Female
Kinesin / metabolism*
Myosins / metabolism*
Ovary / cytology
Palaemonidae / cytology,  metabolism*
Chemical
Reg. No./Substance:
EC 3.6.1.-/Kinesin; EC 3.6.4.1/Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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