Document Detail


Association of high proliferation in adult T-cell leukemia cells with apoptosis, and expression of p53 protein in acute type ATL.
MedLine Citation:
PMID:  18434687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proliferation, apoptosis and p53 protein expression in adult T-cell leukemia (ATL) cells were investigated. Twenty peripheral blood tissue specimens (PBTS) comprising 7 cases of acute type ATL, 7 cases of chronic type ATL and 6 other leukemias were examined by means of antigen retrieval and the polymer method employing anti-Ki67 antigen (MIB-1), anti-cleaved caspase-3, anti-single stranded DNA and three kinds of anti-p53 protein antibodies including DO7. Most acute and chronic cases of ATL included more than 10% MIB-1-positive proliferating leukemia cells and more than 1% cleaved caspase-3-positive apoptotic cells. Some cells which were positive for both MIB-1 and anti-cleaved caspase-3 antibody were observed in acute type ATL. Nuclear deposition of p53 protein labeled by DO7 was often found in acute type (p < 0.05). Within the medium-sized population of ATL cell nuclei, DO7-positive ATL cells had a smaller nuclear area factor (long axis x short axis) than DO7-negative ATL cells. A few proliferating ATL cells entered apoptosis, and the appearance of a subclone of ATL cells with nuclear deposition of p53 protein labeled by DO7 characterized acute type.
Authors:
Jia Wang; Kazuhisa Hasui; Atae Utsunomiya; Xinshan Jia; Takami Matsuyama; Fusayoshi Murata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical and experimental hematopathology : JCEH     Volume:  48     ISSN:  1346-4280     ISO Abbreviation:  J Clin Exp Hematop     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-24     Completed Date:  2008-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101141257     Medline TA:  J Clin Exp Hematop     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Affiliation:
Divsion of Persistent & Oncogenic Viruses (Center for Chronic Viral Diseases), Field of Infection and Immunity, Course of Health Research, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Apoptosis / physiology*
Caspase 3 / biosynthesis
Cell Proliferation*
Female
Humans
Immunohistochemistry
Ki-67 Antigen / biosynthesis
Leukemia, Prolymphocytic, T-Cell / metabolism,  pathology*
Leukemia-Lymphoma, Adult T-Cell / metabolism,  pathology*
Male
Middle Aged
Tumor Suppressor Protein p53 / biosynthesis*
Chemical
Reg. No./Substance:
0/Ki-67 Antigen; 0/Tumor Suppressor Protein p53; EC 3.4.22.-/Caspase 3

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