| Association of granulocyte transmigration with structural and cellular parameters of injury in experimental radiation enteropathy. | |
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MedLine Citation:
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PMID: 9436244 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Inflammatory cells are involved in the pathogenesis of tissue injury through release of cytokines and biologically active compounds. This study used a novel, noninvasive method to assess the association between granulocyte transmigration and structural and molecular changes in radiation enteropathy. A 4 cm loop of rat small intestine was exposed to 0, 2.8, 12, or 23 Gy localized irradiation. Feces was collected in metabolic cages before and 3, 7, 14, 28, and 42 days after irradiation. Granulocyte marker protein (GMP) was measured in buffer extracts of feces by enzyme-linked immunosorbent assay (ELISA). Irradiated and shielded intestine were procured at 2 and 26 weeks and assessed for histopathologic injury [radiation injury score (RIS)], ED-2 positive macrophages, and interleukin-1 alpha (IL-1 alpha) positive cells. Irradiated intestine exhibited characteristic histopathologic alterations and increased numbers of macrophages and IL-1 alpha positive cells. There was a highly significant dose-dependent increase in post-radiation GMP (P < 0.0001). Maximal GMP excretion occurred 3-7 days after irradiation. Six weeks after irradiation, GMP excretion had returned to normal in the 2.8 and 12 Gy groups, but was still 3.5 times higher in the 23 Gy group than in controls. The associations between early GMP excretion and RIS and fibrosis at 26 weeks were highly significant (P < 0.001 and P < 0.0001, respectively). Post-radiation granulocyte transmigration is dose-dependent and correlates with structural and molecular changes, as well as with subsequent chronic injury. The GMP assay is a sensitive, non-invasive indicator of acute intestinal radiation injury and a promising biological predictor of chronic toxicity. Our data underscore the importance of consequential mechanisms in radiation enteropathy. |
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Authors:
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K K Richter; M K Fagerhol; J C Carr; J M Winkler; C C Sung; M Hauer-Jensen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Radiation oncology investigations Volume: 5 ISSN: 1065-7541 ISO Abbreviation: Radiat Oncol Investig Publication Date: 1997 |
Date Detail:
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Created Date: 1998-02-27 Completed Date: 1998-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9437448 Medline TA: Radiat Oncol Investig Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 275-82 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Arkansas for Medical Sciences, Little Rock, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Markers / analysis Blood Proteins / analysis*, biosynthesis Cell Movement / radiation effects* Confidence Intervals Culture Techniques Disease Models, Animal Dose-Response Relationship, Radiation Enzyme-Linked Immunosorbent Assay Feces / chemistry Fibrosis Granulocytes / metabolism, radiation effects* Interleukin-1 / analysis Intestinal Mucosa / metabolism, pathology, radiation effects Intestine, Small / chemistry, pathology, radiation effects* Male Radiation Injuries, Experimental / pathology, physiopathology* Rats Rats, Sprague-Dawley Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Blood Proteins; 0/Interleukin-1 |
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