| Association of cholesterol oxidation and abnormalities in fatty acid metabolism in cystic fibrosis. | |
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MedLine Citation:
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PMID: 19587087 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Disarrangement in fatty acids and oxidative stress are features of cystic fibrosis. Cholesterol is very sensitive to oxidative stress. OBJECTIVES: The objectives were to examine whether cholesterol oxidation products are altered in cystic fibrosis and whether they are associated with fatty acids and with characteristics of the disease state. DESIGN: 7-Ketocholesterol and 7beta-hydroxycholesterol (prototype molecules of free radical-mediated cholesterol oxidation) and the fatty acid profile were assessed by mass spectrometry in patients and in sex- and age-matched control subjects. RESULTS: In a comparison with control subjects, mean (+/-SD) cholesterol oxidation was higher (7-ketocholesterol: 11.31 +/- 5.1 compared with 8.33 +/- 5.5 ng/mL, P = 0.03; 7beta-hydroxycholesterol: 14.5 +/- 6.8 compared with 9.7 +/- 4.1 ng/mL, P = 0.004), total saturated fatty acids were higher (31.90 +/- 1.93% compared with 30.31 +/- 0.98%, P < 0.001), monounsaturated fatty acids were higher (29.14 +/- 3.85% compared with 25.88 +/- 2.94%, P = 0.004), omega-6 (n-6) polyunsaturated fatty acids were lower (34.84 +/- 4.77 compared with 39.68 +/- 2.98%, P < 0.0001), and omega-3 (n-3) polyunsaturated fatty acids were comparable in patients with cystic fibrosis. Oxysterols were inversely associated with 24:0 and 18:2 omega-6 fatty acids but did not correlate with the increased oleic acid or with any of the omega-3 fatty acids. CONCLUSIONS: Cystic fibrosis is characterized by relevant cholesterol oxidation that is associated with an abnormal fatty acid profile. The interplay between oxysterols and fatty acids potentially provides insight into the biological mechanisms that underlie this complex disease. |
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Authors:
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Luigi Iuliano; Roberto Monticolo; Giuseppe Straface; Sabina Zullo; Francesco Galli; Mona Boaz; Serena Quattrucci |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-08 |
Journal Detail:
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Title: The American journal of clinical nutrition Volume: 90 ISSN: 1938-3207 ISO Abbreviation: Am. J. Clin. Nutr. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-21 Completed Date: 2009-09-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376027 Medline TA: Am J Clin Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 477-84 Citation Subset: AIM; IM |
Affiliation:
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Department of Experimental Medicine, Sapienza University of Rome, Italy. luigi.iuliano@uniroma1.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Biological Markers / blood Case-Control Studies Chi-Square Distribution Cholesterol / metabolism* Cystic Fibrosis / metabolism* Fatty Acids / blood*, metabolism Female Humans Hydroxycholesterols / blood Ketocholesterols / blood Male Mass Spectrometry Oxidation-Reduction Oxidative Stress* Statistics, Nonparametric Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Fatty Acids; 0/Hydroxycholesterols; 0/Ketocholesterols; 566-26-7/cholest-5-en-3 beta,7 alpha-diol; 566-28-9/7-ketocholesterol; 57-88-5/Cholesterol |
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