Document Detail

Association between leptin, LH and its receptor and luteinization and progesterone accumulation (P4) in bovine granulosa cell in vitro.
MedLine Citation:
PMID:  18954383     Owner:  NLM     Status:  MEDLINE    
A full understanding of the cellular events that occur during in vitro luteinization of bovine granulosa cells, stimulated by LH and by leptin, is a complex goal that has not been completely achieved. The aim of this work was to study the effects of leptin, LH and leptin + LH on progesterone accumulation (P4) and on the expression of LH receptors (LHR) in bovine granulosa cells in culture. The results confirm that this in vitro model is representative of functional and morphological luteinization/differentiation. The pattern of expression of LHR with time of incubation was an important marker of in vitro luteinization, with 50-90% of cells expressing LHR by 96 h in culture. Cytoplasmic lipidic droplets were highly abundant in granulosa cells, suggesting a sufficient source of precursors for steroid hormone synthesis: P4 accumulation ranged between 40 and 550 ng/ml. In addition, a positive correlation (r = 0.58, p < 0.05) between the expression of LHR and accumulation of P4 throughout the time of incubation was observed. The expression of LHR was inhibited by LH and leptin + LH treatment. In conclusion, we found an inverse modulation between the expression of LHR and the concentration of LH, and the expression of LHR could be regulated by P4 produced by the luteinized granulosa cells. These findings are contributing to elucidate further the panoply of interactions during the differentiation of granulosa cells into luteal cells in vitro.
E Montaño; M Olivera; Z T Ruiz-Cortés
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-13
Journal Detail:
Title:  Reproduction in domestic animals = Zuchthygiene     Volume:  44     ISSN:  1439-0531     ISO Abbreviation:  Reprod. Domest. Anim.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-07-27     Completed Date:  2009-10-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9015668     Medline TA:  Reprod Domest Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  699-704     Citation Subset:  IM    
Grupo Biogénesis, Facultad de Cienias Agragrias, Universidad de Antioquia, Medellín, Colombia.
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MeSH Terms
Cattle / physiology*
Cell Differentiation
Cells, Cultured
Fluorescent Antibody Technique, Indirect
Granulosa Cells / cytology,  drug effects,  metabolism*
Leptin / administration & dosage*
Luteinization / drug effects*
Luteinizing Hormone / administration & dosage*
Progesterone / biosynthesis
Receptors, LH / analysis,  drug effects
Reg. No./Substance:
0/Leptin; 0/Receptors, LH; 57-83-0/Progesterone; 9002-67-9/Luteinizing Hormone

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