Document Detail


Association between chloroquine resistance phenotypes and point mutations in pfcrt and pfmdr1 in Plasmodium falciparum isolates from Thailand.
MedLine Citation:
PMID:  18950599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The relationship between the in vitro susceptibility of Plasmodium falciparum isolates to the quinoline antimalarials chloroquine (CQ), mefloquine (MQ), and quinine (QN), and pfcrt and pfmdr1 gene polymorphisms were investigated. Field isolates (110 samples) were collected from various endemic areas of Thailand throughout 2002-2004. The pfcrt 76T allele was identified in 109 isolates (99.1%) while pfcrt 76K was found in a single (0.9%) isolate. The pfmdr 86N, 86Y, and the combination (86N+86Y) alleles were identified in 83 (75.5%), 22 (20%), and 5 (4.5%) isolates, respectively. The pfmdr1 1042N, 1042D alleles and a mixture (1042N+1042D) of the alleles were found in 94 (85.5%), 12 (10.9%) and 4 (3.6%) isolates, respectively. The pfmdr1 1246Y allele was detected in a single (0.9%) isolate. The pfmdr1 gene polymorphisms (86-1042-1246) was grouped into seven haplotypes as follows: N-N-D (68 isolates; 61.2%), Y-N-D (22 isolates; 19.8%), N-D-D (11 isolates; 9.9%), N-D-Y (1 isolate; 0.9%), N/Y-N-D (4 isolates; 3.6%), N-N/D-D (3 isolates; 2.7%), and N/Y-N/D-D (1 isolate; 0.9%). Eight different combinations of pfcrt-pfmdr1 genotypes were observed. Only one CQ-, MQ- and QN-sensitive isolate was found at the Thai-Laos border and no cases of QN resistance were found in this study.
Authors:
Kanchana Rungsihirunrat; Wanna Chaijareonkul; Aree Seugorn; Kesara Na-Bangchang; Sodsri Thaithong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-30
Journal Detail:
Title:  Acta tropica     Volume:  109     ISSN:  1873-6254     ISO Abbreviation:  Acta Trop.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-02     Completed Date:  2009-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370374     Medline TA:  Acta Trop     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  37-40     Citation Subset:  IM    
Affiliation:
The College of Public Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand. rskanchana@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antimalarials / pharmacology
Chloroquine / pharmacology*
Drug Resistance / genetics
Laos
Membrane Transport Proteins / genetics*
Multidrug Resistance-Associated Proteins / genetics*
Plasmodium falciparum / drug effects,  genetics*,  isolation & purification
Point Mutation*
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Protozoan Proteins / genetics*
Thailand
Chemical
Reg. No./Substance:
0/Antimalarials; 0/Mdr1 protein, Plasmodium falciparum; 0/Membrane Transport Proteins; 0/Multidrug Resistance-Associated Proteins; 0/PfCRT protein, Plasmodium falciparum; 0/Protozoan Proteins; 54-05-7/Chloroquine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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