| Association of apolipoprotein A-II with egg phosphatidylcholine unilamellar vesicles. | |
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MedLine Citation:
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PMID: 6438077 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Interaction of human apolipoprotein A-II (apoA-II) with egg yolk phosphatidylcholine unilamellar vesicles (diameter, 20-26 nm) was studied. ApoA-II bound to the vesicles in a saturable manner and was displaced by apolipoprotein C-III1 (apoC-III1) while bound apoC-III1 was displaced by apoA-II, with apparent deformation of the vesicles, as observed in electronmicrographs. Free apoA-II that had been displaced from the lipid surface was readsorbed to vesicles successively added to the medium. Disruption of the vesicular structure occurred in less than 3% of the vesicles under the experimental conditions. Isothermal binding data were analyzed according to a reversible equilibrium binding model. The dissociation constant, Kd, was 5.8 X 10(-7) M, and the maximum binding level, N, was 1.46 amino acid residues per phospholipid for apoA-II assuming that 70% of the phospholipid is in the outer leaflet of the vesicles, while for apoC-III1 the Kd and N values were 7.8 X 10(-7) M and 1.34 amino acid residues per phospholipid in the outer leaflet, respectively. All these parameters show good agreement with those for triglyceride-phospholipid particles of approximately the same diameter as that of the vesicles (J. Biol. Chem. (1983) 258, 10073-10082). Thus the parameters represent the equilibrium binding of apoA-II to the surface of phosphatidylcholine unilamellar vesicles. |
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Authors:
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S Yokoyama; S Tajima; A Yamamoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 96 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1984 Sep |
Date Detail:
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Created Date: 1985-01-14 Completed Date: 1985-01-14 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 871-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Apolipoprotein A-II Apolipoproteins A / blood* Binding, Competitive Egg Yolk Female Humans Kinetics Lipoproteins, HDL / blood* Liposomes* Microscopy, Electron Phosphatidylcholines / metabolism* Protein Binding |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein A-II; 0/Apolipoproteins A; 0/Lipoproteins, HDL; 0/Liposomes; 0/Phosphatidylcholines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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