Document Detail

Association of Wnt2 and sFRP4 expression in the third trimester placenta in women with severe preeclampsia.
MedLine Citation:
PMID:  23322712     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The Wnt signaling pathway is a conserved pathway and plays a crucial role in regulating trophoblast functions. Abnormal expression of the Wnt pathway may result in the dysfunction of the trophoblast that can contribute to the pathogenesis of preeclampsia (PE). However, published data regarding the association between Wnt pathway and PE in human pregnancy is rare.
OBJECTIVE: The aims of this study were to investigate the expression pattern of Wnt2 and secreted frizzled-related protein 4 (sFRP4) in the third trimester human placenta and to evaluate the relationship between changes in placental Wnt2 and sFRP4 expression and severe PE.
METHODS: The expression of Wnt2 and sFRP4 in normal and severe PE placentas was examined using immunohistochemistry (IHC), real-time polymerase chain reaction, and Western blot.
RESULTS: Compared to the controls, the relative expression of Wnt2 messenger RNA was remarkably downregulated in the PE placentas, while there was no significant difference in sFRP4 between the 2 groups. The IHC indicated that Wnt2 and sFRP4 were expressed predominantly in the villous syncytiotrophoblast and the extravillous trophoblast, whereas Wnt2 in the control group showed higher staining intensity than in the PE group, and sFRP4 in the PE group had a higher staining intensity than in the control group. Furthermore, the results of the Western blots were consistent with the IHC.
CONCLUSIONS: The Wnt signaling pathway was detected in human third trimester placentas, and the decreased placental expression of Wnt2 and increased placental expression of sFRP4 may be associated with the pathogenesis of severe PE.
Zhan Zhang; Lin Zhang; Linlin Zhang; Liting Jia; Peng Wang; Yan Gao
Publication Detail:
Type:  Journal Article     Date:  2013-01-15
Journal Detail:
Title:  Reproductive sciences (Thousand Oaks, Calif.)     Volume:  20     ISSN:  1933-7205     ISO Abbreviation:  Reprod Sci     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-07-17     Completed Date:  2014-01-09     Revised Date:  2014-08-05    
Medline Journal Info:
Nlm Unique ID:  101291249     Medline TA:  Reprod Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  981-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Case-Control Studies
Gene Expression Regulation, Developmental
Placenta / chemistry*
Pre-Eclampsia / diagnosis,  genetics,  metabolism*
Pregnancy Trimester, Third / genetics,  metabolism
Proto-Oncogene Proteins / analysis*,  genetics
RNA, Messenger / analysis
Severity of Illness Index
Wnt Signaling Pathway* / genetics
Wnt2 Protein / analysis*,  genetics
Young Adult
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/RNA, Messenger; 0/SFRP4 protein, human; 0/WNT2 protein, human; 0/Wnt2 Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Singleton-Merten syndrome: An autosomal dominant disorder with variable expression.
Next Document:  Hydroisomerization of Emerging Renewable Hydrocarbons using Hierarchical Pt/H-ZSM-22 Catalyst.